Team:Newcastle/Labwork/25 August 2009

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Revision as of 09:59, 26 August 2009

Meetings Calendar

Stochastic Switch team

Summary

Today we decided we should redo the minipreps from last weeks biobricks using the overnight cultures we set up yesterday. For the miniprep procedure we followed the extra guidelines that Chris advised us about. The final rehydrated plasmid minipreps were put into the -20 freezer in the 'iGEM plasmid minipreps' box. We also did a restriction digest of the two miniprep samples for biobrick C0178 and ran this on a 0.8% agarose gel. This went well, both samples having the fragment sizes expected. Tomorrow we will be carrying out another restriction digest of todays minipreps, however to save time tomorrow, we poured a gel and put it into the fridge ready to load the samples tomorrow. In order to make sure the prepared gel did not dry out, a small amount of 1% TAE was poured onto the gel, which was wrapped in cling film, clearly labelled and put into the fridge.

Restriction digest

Today we did restriction digest for a miniprep prepared for BBa_C0178. We prepared DNa ladder to load to the gels. At the end of the day, we prepared our gel to run tomorrow.We followed the restriction digest protocol for our two miniprep prepared for BBa_C0178 and used the followings to prepare the solutions.

H20, 6.5 ul
10x Buffer, 2ul
DNA from minipreps, 10ul
EcoRI, 0.5 ul
PstI, 0.5 ul
BSA, 0.5 ul (It was diluted 100 times)

We gave a quick pulse to mix everything in the tubes before we put them into 37C incubator for an hour.

Running the gel

Metal container team had already prepared the gel for us. We placed the gel tray into the gel electrophoresis device and top it up with 1xTAE buffer

20 – 21 June 2009 - Europe workshop (London)
23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
23 October Practice Presentation (Newcastle)
23 October T-shirts are ready
27 October Practice Presentation (Sunderland)
27 October Poster is ready
30 October – 2 November 2009 - Jamboree (Boston)

Newcastle iGEM Twitter
[http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
[http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]

@@ Line 4: / Line 4: @@
 ==<u>Stochastic Switch team</u>==
 ===Summary===
-Today we did restriction digest for a miniprep prepared for BBa_C0178. We also did minipreps for other six biobricks(....) from last week. We prepared DNa ladder to load to the gels. At the end of the day, we prepared our gel to run tomorrow.
+Today we decided we should redo the minipreps from last weeks biobricks using the overnight cultures we set up yesterday. For the miniprep procedure we followed the extra guidelines that Chris advised us about. The final rehydrated plasmid minipreps were put into the -20 freezer in the 'iGEM plasmid minipreps' box. We also did a restriction digest of the two miniprep samples for biobrick C0178 and ran this on a 0.8% agarose gel. This went well, both samples having the fragment sizes expected.
+Tomorrow we will be carrying out another restriction digest of todays minipreps, however to save time tomorrow, we poured a gel and put it into the fridge ready to load the samples tomorrow.
+In order to make sure the prepared gel did not dry out, a small amount of 1% TAE was poured onto the gel, which was wrapped in cling film, clearly labelled and put into the fridge.
 ===Restriction digest===
-We followed the restriction digest protocol for our two miniprep prepared for BBa_C0178 and used the followings to prepare the solutions.
+Today we did restriction digest for a miniprep prepared for BBa_C0178.  We prepared DNa ladder to load to the gels. At the end of the day, we prepared our gel to run tomorrow.We followed the restriction digest protocol for our two miniprep prepared for BBa_C0178 and used the followings to prepare the solutions.
 * H20, 6.5 ul
 * 10x Buffer, 2ul