EPF-Lausanne/25 August 2009

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Revision as of 11:52, 2 September 2009

Wet Lab

Miniprep:

Made according to the protocol of Aisgen Miniprep Kit Elution in 50 ul. The RD2 #5 plasmids prep as some that have already been prepared will be sent for sequencing this afternoon.

Mediums

New medium were made to grow the double-transformed DH5 a (LovTap BB and RO2) -> should be without thy.

M9 was redone, pH adjusted with amino acid and filtred. for 1L:

5g glucose

6g Na2PO4

3g KH2PO4

1g NH4Cl

0.5g NaCl

0.12g MgSO4

0.01g CaCl2

Trp Operon synthesis

Using the Klenow fragments protocol and primers:£

Trp operon Rev

Trp operon Fwd

**Parts&Characteristics**
	2x Trp	1x Negative Control
NEB 2 (10x)	Terminator	A	13D (1)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]	Promoter LacI	A	1D (1)
[http://partsregistry.org/Part:BBa_B0030 BBa_B0030]	RBS	A	1H (1)
[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]	RBS-GFP-TER	A	12M (1)
[http://partsregistry.org/Part:BBa_I13507 BBa_I13507]	RBS-mRFP-TER	A	22O (1)
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002]	pTetR-RBS	A	13B (1)

People in the lab

Nath, Basile, Gab, Christian

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-[http://partsregistry.org/Part:BBa_B0010 BBa_B0010]
+NEB 2 (10x)
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