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Team:Groningen/Notebook/5 September 2009
From 2009.igem.org
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===Metal Accumulation=== | ===Metal Accumulation=== | ||
| - | Plasmid isolation of [[Team:Groningen/Notebook/24_August_2009#Metal_Accumulation| SmtA-1]] ON culture | + | ''''Colony PCR'''' |
| + | <center>[[Image:Colony_PCR_of_SmtA-1_-_05september2009.jpg|500px]]</center><BR> | ||
| + | Colony PCR of [[Team:Groningen/Notebook/24_August_2009#Metal_Accumulation|SmtA colony #1]] using Biobrick VF2/VR primers (lane 1) and RBSforward and SmtAreverse primers (lane 2). Probably a different RBS primer was used, explaining the lack of product. SmtA (including RBS) is 678 bp, PCR product using VF2/VR primers should yield a band of 989 bp. Left and right are both the same 1% agarose (1xTBE) gel on different time points.<BR> | ||
| + | Plasmid isolation of [[Team:Groningen/Notebook/24_August_2009#Metal_Accumulation| SmtA-1]] ON culture | ||
===Vectors=== | ===Vectors=== | ||
Revision as of 15:13, 5 September 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
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Wet
GVP Cluster
| E. coli TOP10 Colonies | Name | Plasmid | Date of plating | Antibiotics |
|---|---|---|---|---|
| pCueO-GVP conc. | 4september2009 | Kanamycin/IPTG | ||
| pZntR-GVP conc. | 4september2009 | Kanamycin/IPTG | ||
| pArsR-GVP conc. | 4september2009 | Kanamycin/IPTG | ||
| pLac-GVP conc. | 4september2009 | Kanamycin/IPTG | ||
| (7K) | 4september2009 | Kanamycin/IPTG | ||
| (7K) conc. | 4september2009 | Kanamycin/IPTG | ||
| (7C) | 4september2009 | Kanamycin/IPTG | ||
| (7C) conc. | 4september2009 | Kanamycin/IPTG |
Plates with colonies were stored in fridge, the red indicates a red colony. Plates without colonies were left on the bench.
Transporters
Metal Accumulation
'Colony PCR'
Colony PCR of SmtA colony #1 using Biobrick VF2/VR primers (lane 1) and RBSforward and SmtAreverse primers (lane 2). Probably a different RBS primer was used, explaining the lack of product. SmtA (including RBS) is 678 bp, PCR product using VF2/VR primers should yield a band of 989 bp. Left and right are both the same 1% agarose (1xTBE) gel on different time points.
Plasmid isolation of SmtA-1 ON culture
Vectors
Dry
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