Team:UNICAMP-Brazil/Notebooks/August 15

(Difference between revisions)

Revision as of 18:44, 7 September 2009

Today we started the construction of our first biobrick. After quantificate the miniprep's products (from 11/Aug) we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.

After the digestion we isolated the target bands from the agarose gel, according to the protocol X.

The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.

Raíssa

@@ Line 1: / Line 1: @@
-== YeastGuard: RBS and CFP plasmid digestion ==
+{{:Team:UNICAMP-Brazil/inc_topo}}
+{{:Team:UNICAMP-Brazil/inc calendar}}
-- Today we started the construction of our first biobrick. After quantificate the mini-prep's products (from 11/Aug) we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.
+__NOTOC__
-- After the digestion we isolated the target bands from the agarose gel, according to the protocol X.
+==''' YeastGuard '''==
-- The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.
+====RBS and CFP plasmid digestion====
-'''Raíssa'''
+* Today we started the construction of our first biobrick. After quantificate the miniprep's products (from 11/Aug) we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.
+* After the digestion we isolated the target bands from the agarose gel, according to the protocol X.
+* The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.
+''Raíssa''
 {{:Team:UNICAMP-Brazil/inc_rodape}}