Team:UNICAMP-Brazil/Notebooks/August 17

(Difference between revisions)

Revision as of 18:46, 7 September 2009

The RBS band purification performed on August 15 didn't work, so we digested the plasmid again. Curiously the digested band was a little bigger than we expected, so we decided to check over it's size on Registry and we found that we ressuspended the wrong biobrick!!! Fortunately it's purification didn't work before! =)

After this finding we checked the other biobricks and ressuspended the correct RBS. We transformed it in E. coli, plated and grew it in liquid media.

Raíssa

@@ Line 1: / Line 1: @@
-== YeastGuard: Trying to purify the RBS digested fragment ==
+{{:Team:UNICAMP-Brazil/inc_topo}}
+{{:Team:UNICAMP-Brazil/inc calendar}}
-- The RBS band purification performed on August 15 didn't work, so we digested the plasmid again. Curiously the digested band was a little bigger than we expected, so we decided to check over it's size on Registry and we found that we ressuspended the wrong biobrick!!! Fortunatly it's purification didn't work before! =)
+__NOTOC__
-- After this finding we checked the other biobricks and ressuspended the correct RBS one.  We transformed it in ''E. coli'', plated and grew it in liquid media.
+==''' YeastGuard '''==
-'''Raíssa'''
+====Trying to purify the RBS digested fragment====
+* The RBS band purification performed on August 15 didn't work, so we digested the plasmid again. Curiously the digested band was a little bigger than we expected, so we decided to check over it's size on Registry and we found that we ressuspended the wrong biobrick!!! Fortunately it's purification didn't work before! =)
+* After this finding we checked the other biobricks and ressuspended the correct RBS. We transformed it in ''E. coli'', plated and grew it in liquid media.
+''Raíssa''
 {{:Team:UNICAMP-Brazil/inc_rodape}}