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Team:UNICAMP-Brazil/Notebooks/July 20
From 2009.igem.org
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{{:Team:UNICAMP-Brazil/inc calendar}} | {{:Team:UNICAMP-Brazil/inc calendar}} | ||
| - | + | __NOTOC__ | |
| - | + | ==''' MicroGuards '''== | |
| - | == | + | ====Primer design==== |
| - | After the recovery of F plasmid (July 16th), we performed a digestion of the mini-prep's product, in order to assure that we indeed recover the plasmid itself. | + | * During the week from July 20th to 25th we designed all primers that we´re going to use in our project. All the sequences are showed on July 25th notebook. |
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| + | __NOTOC__ | ||
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| + | ==''' ColiGuard '''== | ||
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| + | ==== Restriction Assay==== | ||
| + | |||
| + | * After the recovery of F plasmid (July 16th), we performed a digestion of the mini-prep's product, in order to assure that we indeed recover the plasmid itself. | ||
We did this assay by using two restriction enzymes: Eco RI and Cla I. We followed protocol nº X, without modifications. | We did this assay by using two restriction enzymes: Eco RI and Cla I. We followed protocol nº X, without modifications. | ||
We then compared the pattern provided us by pDRAW software with an eletrophoresis gel of the digestion product. | We then compared the pattern provided us by pDRAW software with an eletrophoresis gel of the digestion product. | ||
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| - | + | ''Gabriel, Marcelo and Victor.'' | |
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Revision as of 16:41, 7 September 2009
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