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Team:UNICAMP-Brazil/Notebooks/July 24
From 2009.igem.org
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Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process). | Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process). | ||
| - | We performed minipreps from R plasmid-containing cultures, according to Protocol 2 | + | We performed minipreps from R plasmid-containing cultures, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2] |
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As we did for F plasmid, we were also able to recover the R plasmid. | As we did for F plasmid, we were also able to recover the R plasmid. | ||
Revision as of 23:08, 3 October 2009
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