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Team:UNICAMP-Brazil/Notebooks/August 28
From 2009.igem.org
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====Terminator biobrick==== | ====Terminator biobrick==== | ||
| - | * | + | * Colony PCR |
The colony PCR was made to confirm the presence of terminator transformants, because the antibiotic resistence is not working to any antibiotics. | The colony PCR was made to confirm the presence of terminator transformants, because the antibiotic resistence is not working to any antibiotics. | ||
We plated the the cells in LB, and picked some colonies with a toothpick, inoculate on a backup LB plate and put the toothpick on a tube with 20µl H2O, then, denature this cells 95°C, 5 minutes and heat shock on ice 5 minutes. Spin the tubes and use 1µl to make the PCR (see protocol). | We plated the the cells in LB, and picked some colonies with a toothpick, inoculate on a backup LB plate and put the toothpick on a tube with 20µl H2O, then, denature this cells 95°C, 5 minutes and heat shock on ice 5 minutes. Spin the tubes and use 1µl to make the PCR (see protocol). | ||
| - | + | * Preparing for Miniprep | |
We also innoculated ten colonies transformed with the terminator biobrick to do the miniprep tomorrow. | We also innoculated ten colonies transformed with the terminator biobrick to do the miniprep tomorrow. | ||
Revision as of 19:29, 7 September 2009
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