Team:UNICAMP-Brazil/Notebooks/September 4

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(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' MicroGuards '''== ====Electrocompetent ''E. coli''==== Today we prepared electrocompetent ''E. ...)
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Today we prepared electrocompetent ''E. coli'' to use in our transformations according to Protocol 4 (see protocols section).
Today we prepared electrocompetent ''E. coli'' to use in our transformations according to Protocol 4 (see protocols section).
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==''' ColiGuards '''==
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==== Cre-Recombinase without ATG ====
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Today we started the construction of Cre-Recombinase without ATG, in order to made a new biobrick involving this construction.  We ressuspended the biobrick of Cre-Recombinase: BBa_J61047  and transformed in E. coli DH10B eletrocompetent cells. We then plated on LB Amp plate, according to information from registry page.
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Víctor
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 20:39, 26 September 2009

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MicroGuards

Electrocompetent E. coli

Today we prepared electrocompetent E. coli to use in our transformations according to Protocol 4 (see protocols section).

ColiGuards

Cre-Recombinase without ATG

Today we started the construction of Cre-Recombinase without ATG, in order to made a new biobrick involving this construction. We ressuspended the biobrick of Cre-Recombinase: BBa_J61047 and transformed in E. coli DH10B eletrocompetent cells. We then plated on LB Amp plate, according to information from registry page.

Víctor