Team:UNICAMP-Brazil/Notebooks/September 5

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Revision as of 21:26, 12 September 2009

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MicroGuards

We made more 22 LB-AMP plates.

Taís

YeastGuard

Terminator Biobrick

We purified the Terminator digestion from agarose gel using Pure Link Kit.

ColiGuard

finO and finP Isolation from R Plasmid

After the successful recovery of the R plasmid (July 24th), we could start working on the isolation of the finO and finP sequences from this plasmid. We performed a PCR using the primers we designed in July 25th, and then we ran an eletrophoresis gel with the products, in order to confirm that we indeed amplified the correct fragments.

As shown is the photo, the finO's PCR product reached about 600 pb, and the finP's PCR product reached about 120 bp. The size reached by both products correspond to the estimated lenght of the finO and finP sequences, suggesting that we amplified the correct fragments, and that we could sucessful isolate both sequences from the R plasmid.

Bruno and Marcelo

@@ Line 15: / Line 15: @@
 We purified the Terminator digestion from agarose gel using Pure Link Kit.
+==''' ColiGuard '''==
+====finO and finP Isolation from R Plasmid====
+* After the successful recovery of the R plasmid (July 24th), we could start working on the isolation of the finO and finP sequences from this plasmid. We performed a PCR using the primers we designed in July 25th, and then we ran an eletrophoresis gel with the products, in order to confirm that we indeed amplified the correct fragments.
+[[Image:isolar_finOP.JPG‎ |center|‎]]
+As shown is the photo, the finO's PCR product reached about 600 pb, and the finP's PCR product reached about 120 bp. The size reached by both products correspond to the estimated lenght of the finO and finP sequences, suggesting that we amplified the correct fragments, and that we could sucessful isolate both sequences from the R plasmid.
+''Bruno and Marcelo''
 {{:Team:UNICAMP-Brazil/inc_rodape}}