Team:UNICAMP-Brazil/Notebooks/September 5

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====finO and finP Isolation from R Plasmid====
====finO and finP Isolation from R Plasmid====
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* After the successful recovery of the R plasmid (July 24th), we could start working on the isolation of the finO and finP sequences from this plasmid. We performed a PCR using the primers we designed in July 25th, and then we ran an eletrophoresis gel with the products, in order to confirm that we indeed amplified the correct fragments.
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* After the successful recovery of the R plasmid (July 24th), we could start working on the isolation of the finO and finP sequences from this plasmid. We performed a PCR using the primers we designed on July 25th, and then we ran an eletrophoresis gel with the products, in order to confirm that we indeed amplified the correct fragments.

Revision as of 14:40, 13 September 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
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MicroGuards

We made more 22 LB-AMP plates.

Taís

YeastGuard

Terminator Biobrick

We purified the Terminator digestion from agarose gel using Pure Link Kit.

ColiGuard

finO and finP Isolation from R Plasmid

  • After the successful recovery of the R plasmid (July 24th), we could start working on the isolation of the finO and finP sequences from this plasmid. We performed a PCR using the primers we designed on July 25th, and then we ran an eletrophoresis gel with the products, in order to confirm that we indeed amplified the correct fragments.


‎

As shown is the photo, the finO's PCR product reached about 600 bp, and the finP's PCR product reached about 120 bp. The size reached by both products correspond to the estimated lenght of the finO and finP sequences, suggesting that we amplified the correct fragments, and that we sucessful isolated both sequences from the R plasmid.

Marcelo




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