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Team:UNICAMP-Brazil/Notebooks/August 15
From 2009.igem.org
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====RBS and CFP plasmid digestion==== | ====RBS and CFP plasmid digestion==== | ||
| - | * Today we started the construction of our first biobrick. After quantificate the miniprep's products (from 11/Aug) we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter. | + | *<p style=”text-align:justify;”>Today we started the construction of our first biobrick. After quantificate the miniprep's products (from 11/Aug) we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.</p> |
| - | * After the digestion we isolated the target bands from the agarose gel, according to the | + | *<p style=”text-align:justify;”>After the digestion we isolated the target bands from the agarose gel, according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Purification_of_DNA_fragments_from_agarose_gels Protocol 7].</p> |
| - | * The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today. | + | *<p style=”text-align:justify;”>The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.</p> |
''Raíssa'' | ''Raíssa'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Revision as of 23:00, 3 October 2009
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