Team:UNICAMP-Brazil/Notebooks/August 29

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YeastGuard

Lysozyme

Material

We found a farm that creates chicken for food production, and they have donated us the organs we needed to make the RNA extraction of Gallus gallus: the blood, the spleen and the oviduct.

RNA extraction

We used the Trizol protocol for the three tissues (Protocol 8). We obtained pellets from all tissues! Hope their concentration and quality are ok! =]

Gleidson, Raíssa, Taís and Sula

Terminator Biobrick

Today we perfomed miniprep of the Terminator Biobrick transformed E. coli, according to Protocol 2.

Wesley

@@ Line 12: / Line 12: @@
 [[Image:chicken_run_4.jpg|right]]
 =====RNA extraction=====
-We used the Trizol protocol for the three tissues (Protocol 8).
+We used the Trizol protocol for the three tissues ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/RNA_isolation_from_%27Gallus_gallus%27_spleen%2C_blood_and_oviduct Protocol 8]).
 We obtained pellets from all tissues! Hope their concentration and quality are ok! =]
@@ Line 23: / Line 22: @@
 ====Terminator Biobrick====
-Today we perfomed miniprep of the Terminator Biobrick transformed ''E. coli'', according to Protocol 2 (see protocols section).
+Today we perfomed miniprep of the Terminator Biobrick transformed ''E. coli'', according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2].
 ''Wesley''
 {{:Team:UNICAMP-Brazil/inc_rodape}}