Team:UNICAMP-Brazil/Notebooks/September 22

(Difference between revisions)

Revision as of 18:04, 27 September 2009

The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!
Now that the F plasmid was obtained, the next step is its recircularization.

Gabriel

We inoculated those colonies that actually resulted in a expected amplification size fragment after the miniprep procedure (recent assays), separately into liquid LB-AMP media, in order to repeat the miniprep, aiming in reducing the amount of genomic DNA extracted together.

Marcelo

Revision as of 18:02, 27 September 2009 (view source) Mbassalo (Talk \| contribs) ← Older edit		Revision as of 18:04, 27 September 2009 (view source) Mbassalo (Talk \| contribs) (→finO and finP - Still Trying to Confirm our Biobricks) Newer edit →
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	====finO and finP - Still Trying to Confirm our Biobricks====		====finO and finP - Still Trying to Confirm our Biobricks====

-	* We ~~innoculated~~ those colonies that actually resulted in a expected amplification size fragment after the miniprep procedure (recent assays), separately into liquid LB-AMP media, in order to repeat the miniprep, aiming in reducing the amount of genomic DNA extracted together.	+	* We inoculated those colonies that actually resulted in a expected amplification size fragment after the miniprep procedure (recent assays), separately into liquid LB-AMP media, in order to repeat the miniprep, aiming in reducing the amount of genomic DNA extracted together.