Team:UNICAMP-Brazil/Notebooks/September 22

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==''' ColiGuard '''==
==''' ColiGuard '''==
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====Electroelution verification====
====Electroelution verification====
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*The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!
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*<p style=”text-align:justify;”>The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!</p>
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*Now that the F plasmid was obtained, the next step is its recircularization.
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*<p style=”text-align:justify;”>Now that the F plasmid was obtained, the next step is its recircularization.</p>
''Gabriel''
''Gabriel''
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====finO and finP - Still Trying to Confirm our Biobricks====
====finO and finP - Still Trying to Confirm our Biobricks====
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* We inoculated those colonies that actually resulted in a expected amplification size fragment after the miniprep procedure (recent assays), separately into liquid LB-AMP media, in order to repeat the miniprep, aiming in reducing the amount of genomic DNA extracted together.
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*<p style=”text-align:justify;”>We inoculated those colonies that actually resulted in a expected amplification size fragment after the miniprep procedure (recent assays), separately into liquid LB-AMP medium, in order to repeat the miniprep, aiming in reducing the amount of genomic DNA extracted together.</p>
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''Marcelo''
''Marcelo''

Revision as of 21:45, 3 October 2009

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ColiGuard

Electroelution verification

  • The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!

  • Now that the F plasmid was obtained, the next step is its recircularization.

Gabriel

finO and finP - Still Trying to Confirm our Biobricks

  • We inoculated those colonies that actually resulted in a expected amplification size fragment after the miniprep procedure (recent assays), separately into liquid LB-AMP medium, in order to repeat the miniprep, aiming in reducing the amount of genomic DNA extracted together.

Marcelo





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