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Team:UNICAMP-Brazil/Notebooks/September 27
From 2009.igem.org
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{{:Team:UNICAMP-Brazil/inc calendar}} | {{:Team:UNICAMP-Brazil/inc calendar}} | ||
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==''' ColiGuard '''== | ==''' ColiGuard '''== | ||
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====Inoculation of Yesterday's Ressuspended and Transformed Biobricks==== | ====Inoculation of Yesterday's Ressuspended and Transformed Biobricks==== | ||
| - | * We selected 4 colonies from each plate containing one of yesterday's transformed biobricks, and inoculated them in liquid LB-AMP | + | *<p style=”text-align:justify;”>We selected 4 colonies from each plate containing one of yesterday's transformed biobricks, and inoculated them in liquid LB-AMP medium.</p> |
| - | * Those inocula were grew for an O/N period at 37ºC (250 rpm). | + | *<p style=”text-align:justify;”>Those inocula were grew for an O/N period at 37ºC (250 rpm).</p> |
''Marcelo'' | ''Marcelo'' | ||
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====Digestion of pTet and Double Terminator==== | ====Digestion of pTet and Double Terminator==== | ||
| - | * We pretend to ligate the parts which we are recovering right now (started yesterday) onto biobricks BBa_R0040 (pTet) and BBa_B0015 (double terminator), using it's own vector. | + | *<p style=”text-align:justify;”>We pretend to ligate the parts which we are recovering right now (started yesterday) onto biobricks BBa_R0040 (pTet) and BBa_B0015 (double terminator), using it's own vector.</p> |
| - | * Those biobricks were already recovered on August 10th. | + | *<p style=”text-align:justify;”>Those biobricks were already recovered on August 10th.</p> |
| - | * BBa_R0040 was digested with SpeI and PstI, once we intend to add a fragment in front of it (downstream). As for BBa_B0010, the digestion was realized with EcoRI and Xba restriction enzymes, once we need to insert a fragment behind it's part (upstream). | + | *<p style=”text-align:justify;”>BBa_R0040 was digested with SpeI and PstI, once we intend to add a fragment in front of it (downstream). As for BBa_B0010, the digestion was realized with EcoRI and Xba restriction enzymes, once we need to insert a fragment behind it's part (upstream).</p> |
| - | * Digestion lasted 3 hours. | + | *<p style=”text-align:justify;”>Digestion lasted 3 hours.</p> |
''Marcelo'' | ''Marcelo'' | ||
| + | ==''' YeastGuard '''== | ||
| + | ====Dephosphorylation - CIAP test==== | ||
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| + | *<p style=”text-align:justify;”>The ''E.coli'' didn´t grow in any of the plates. =[ We think that we didn´t plate an enough volume of bacteria. So we decided to replate a larger volume. We hope to find colonies tomorrow!</p> | ||
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| + | ''Raíssa and Taís'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Revision as of 21:21, 3 October 2009
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