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Team:UNICAMP-Brazil/Notebooks/September 26
From 2009.igem.org
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====New Strategy / finO and finP==== | ====New Strategy / finO and finP==== | ||
| - | * We are truly disappointed that we still couldn't make our finO and finP biobricks. We found a lot of issues that must be solved in order to achieve this objective, and we are totally running out of time =( | + | *<p style=”text-align:justify;”>We are truly disappointed that we still couldn't make our finO and finP biobricks. We found a lot of issues that must be solved in order to achieve this objective, and we are totally running out of time =(</p> |
| - | * We decided that we are going to leave finO and finP aside for a while, and we will focus on constructing our modified version of Paris 2007 Team's biobricks. | + | *<p style=”text-align:justify;”>We decided that we are going to leave finO and finP aside for a while, and we will focus on constructing our modified version of Paris 2007 Team's biobricks.</p> |
| - | * We made a whole schedule for that, and we hope we would complete it in a week tops. The next step would be it's insertion into genomic DNA by homologous recombination. | + | *<p style=”text-align:justify;”>We made a whole schedule for that, and we hope we would complete it in a week tops. The next step would be it's insertion into genomic DNA by homologous recombination.</p> |
''Marcelo'' | ''Marcelo'' | ||
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====Recovering More Biobricks==== | ====Recovering More Biobricks==== | ||
| - | * We started our "new strategy" (related to modifying Paris 2007 team's biobricks) by recovering some biobricks that had now became useful for our project. We ressuspended biobricks BBa_E0840 (GFP device), BBa_I718017 (lox71), BBa_J61000 (chloramphenicol resistance cassette) and BBa_I718016 (lox66), and then transfomed them into electrocompetent E. coli cells, strain DH10B, according to protocol 3 (see Protocols section). | + | *<p style=”text-align:justify;”>We started our "new strategy" (related to modifying Paris 2007 team's biobricks) by recovering some biobricks that had now became useful for our project. We ressuspended biobricks BBa_E0840 (GFP device), BBa_I718017 (lox71), BBa_J61000 (chloramphenicol resistance cassette) and BBa_I718016 (lox66), and then transfomed them into electrocompetent E. coli cells, strain DH10B, according to protocol 3 (see Protocols section).</p> |
| - | * The transformed cells were plated in LB-AMP media, and were grown for an O/N period. | + | *<p style=”text-align:justify;”>The transformed cells were plated in LB-AMP media, and were grown for an O/N period.</p> |
''Marcelo and Victor'' | ''Marcelo and Victor'' | ||
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====Dephosphorylation - CIAP test==== | ====Dephosphorylation - CIAP test==== | ||
| - | *Today we performed 5' dephosphorylation of a biobrick vector (previously digested with XbaI and SpeI) with CIAP (Protocol 10) in order to test the reaction efficiency and compare with SAP protocol (Protocol 9). | + | *<p style=”text-align:justify;”>Today we performed 5' dephosphorylation of a biobrick vector (previously digested with XbaI and SpeI) with CIAP (Protocol 10) in order to test the reaction efficiency and compare with SAP protocol (Protocol 9). |
| - | We did two ligation reactions, one using the phosphorylated vector (control) and another using the dephosphorylated (Protocol 11). | + | We did two ligation reactions, one using the phosphorylated vector (control) and another using the dephosphorylated (Protocol 11).</p> |
| - | *We transformed the electrocompetent E. coli (protocol 3) with the ligations and plated in LB+Amp+Kan media. | + | *<p style=”text-align:justify;”>We transformed the electrocompetent E. coli (protocol 3) with the ligations and plated in LB+Amp+Kan media. </p> |
''Raíssa and Taís'' | ''Raíssa and Taís'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Revision as of 21:23, 3 October 2009
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