Team:UNICAMP-Brazil/Notebooks/October 1

MicroGuards

Preparation of electrocompetent E. coli

• Today we prepared more electrocompetent E. coli to use in our transformations according to Protocol 4 (see protocols section).

Marcos and Taís

YeastGuard

New biobricks - "screening" by miniprep and digestion

- Today we did the miniprep using the rest of the transformed E. coli that was not plated in LB+AMP media. After that we digested the miniprep with Xba1, in order to find the band with the correct molecular weight (expected for the inserts connected to the vector). Unfortunately we only found one band with the wrong molecular weight.

GEL

JEN1 ORF

- The concentration of the new part JEN1ORF wasn´t enough for the ligation reactions. So we repeated the PCR and today we digested it with Xba1 and Spe1.

Terminator Biobrick

- We digested the terminator briobrick with Xba1 and Pst1.

Taís