Team:UNICAMP-Brazil/Notebooks/October 1
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====New biobricks - "screening" by miniprep and digestion==== | ====New biobricks - "screening" by miniprep and digestion==== | ||
- | - Today we did the miniprep using the rest of the transformed ''E. coli'' that was not plated in LB+AMP media. After that we digested the miniprep with ''Xba''1, in order to find the band with the correct molecular weight (expected for the inserts connected to the vector). Unfortunately we only found one band with the wrong molecular weight. | + | **<p style=”text-align:justify;”>Today we did the miniprep using the rest of the transformed ''E. coli'' that was not plated in LB+AMP media. After that we digested the miniprep with ''Xba''1, in order to find the band with the correct molecular weight (expected for the inserts connected to the vector). Unfortunately we only found one band with the wrong molecular weight.</p> |
GEL | GEL | ||
====JEN1 ORF==== | ====JEN1 ORF==== | ||
- | - The concentration of the new part JEN1ORF wasn´t enough for the ligation reactions. So we repeated the PCR and today we digested it with ''Xba''1 and ''Spe''1. | + | **<p style=”text-align:justify;”>The concentration of the new part JEN1ORF wasn´t enough for the ligation reactions. So we repeated the PCR and today we digested it with ''Xba''1 and ''Spe''1.</p> |
====Terminator Biobrick==== | ====Terminator Biobrick==== | ||
- | - We digested the terminator briobrick with ''Xba''1 and ''Pst''1. | + | **<p style=”text-align:justify;”>We digested the terminator briobrick with ''Xba''1 and ''Pst''1.</p> |
''Taís'' | ''Taís'' |
Revision as of 21:13, 3 October 2009
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