Team:UNICAMP-Brazil/Notebooks/September 22
From 2009.igem.org
Line 19: | Line 19: | ||
''Marcelo'' | ''Marcelo'' | ||
+ | ====CeaB and CeB: Previous treatment, ligation and transformation==== | ||
+ | *<p style=”text-align:justify;”>In order to avoid recircularization of BBa_B0015 plasmid, we tried it with alkaline phosphatase ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/SAP_Dephosphorylation protocol 9]) . After, we started the ligation reaction of this unphosphorylated plasmid with CeaB and CeiB respectively. In view of this dephosphorylation uses buffer salt that could be interfering in the next transformation, we performed a dyalisis so that get samples free salts. At last, we transformated competent E. coli (according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation protocol 3]) and plated the respective plates. We expect great results for tomorrow!!!. | ||
+ | |||
+ | Luige & Ane | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} |
Revision as of 00:22, 21 October 2009
|