Team:UNICAMP-Brazil/Notebooks/September 5
From 2009.igem.org
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''Taís'' | ''Taís'' | ||
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==''' ColiGuard '''== | ==''' ColiGuard '''== | ||
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==== Cre-Recombinase ==== | ==== Cre-Recombinase ==== | ||
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====finO and finP Isolation from R Plasmid==== | ====finO and finP Isolation from R Plasmid==== | ||
- | * After the successful recovery of the R plasmid (July 24th), we could start working on the isolation of the finO and finP sequences from this plasmid. We performed a PCR using the primers we designed on July 25th, and then we ran an eletrophoresis gel with the products, in order to confirm that we indeed amplified the correct fragments. | + | *<p style=”text-align:justify;”>After the successful recovery of the R plasmid (July 24th), we could start working on the isolation of the finO and finP sequences from this plasmid. We performed a PCR using the primers we designed on July 25th, and then we ran an eletrophoresis gel with the products, in order to confirm that we indeed amplified the correct fragments.</p> |
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''Marcelo'' | ''Marcelo'' | ||
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+ | ==''' YeastGuard '''== | ||
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+ | ====Terminator Biobrick==== | ||
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+ | *We purified the Terminator digestion from agarose gel using Pure Link Kit. | ||
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{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} |
Revision as of 22:49, 3 October 2009
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