Team:UNICAMP-Brazil/Notebooks/July 24

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==== R Plasmid Minipreps ====
==== R Plasmid Minipreps ====
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Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
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*Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
We performed minipreps from R plasmid-containing cultures, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]
We performed minipreps from R plasmid-containing cultures, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]
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Revision as of 20:21, 10 October 2009

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R Plasmid Minipreps

  • Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).

We performed minipreps from R plasmid-containing cultures, according to Protocol 2 . As we did for F plasmid, we were also able to recover the R plasmid.

Gabriel, Marcelo and Victor.





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