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EPF-Lausanne/13 October 2009
From 2009.igem.org
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==Wet Lab== | ==Wet Lab== | ||
| + | Clone LOVTAP into a an iGEM plasmid | ||
| + | LOVTAP = PCR product from 12 Oct 09 ( PCR on plsmid with LOVTAP-Term) | ||
| + | Plasmid = pSB1A3 ( Ampicilin resistance) | ||
| + | Digestion | ||
| + | |||
| + | Check digestion product with agarose gel | ||
| + | |||
| + | Purification | ||
| + | |||
| + | Ligation ( 1h30 ) | ||
| + | |||
| + | Transformation on LB agar plate | ||
==People in the lab== | ==People in the lab== | ||
Revision as of 09:58, 14 October 2009
Contents |
Wet Lab
Clone LOVTAP into a an iGEM plasmid LOVTAP = PCR product from 12 Oct 09 ( PCR on plsmid with LOVTAP-Term) Plasmid = pSB1A3 ( Ampicilin resistance)
Digestion
Check digestion product with agarose gel
Purification
Ligation ( 1h30 )
Transformation on LB agar plate
People in the lab
