Team:UNICAMP-Brazil/Notebooks/July 24

(Difference between revisions)

Revision as of 19:46, 19 October 2009

Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
We performed minipreps from R plasmid-containing cultures, according to Protocol 2.
We then ran an agarose gel with miniprep's sample, also running a few F plasmid's sample as a band-size control.
As we did for F plasmid, we were also able to recover the R plasmid.

Gabriel, Marcelo and Victor.

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 ==== R Plasmid Minipreps ====
-*Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
+* Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
-*We performed minipreps from R plasmid-containing cultures, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2].
+* We performed minipreps from R plasmid-containing cultures, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2].
-As we did for F plasmid, we were also able to recover the R plasmid.
+* We then ran an agarose gel with miniprep's sample, also running a few F plasmid's sample as a band-size control.
+* As we did for F plasmid, we were also able to recover the R plasmid.
 ''Gabriel, Marcelo and Victor.''