Team:UNICAMP-Brazil/Notebooks/September 15

ColiGuard

F plasmid's cultures miniprep

• The cultures grew well and were used to perform the miniprep for F plasmid's extraction, according to Protocol 2.

• The resulting product of the performed miniprep was applied to an 0,8% agarose gel to verify if it went well. Two bands showed up in the gel, with the largest appearing to be a 23 kb fragment. The lower molecular weight fragment, showing to be approximately a 21 kb fragment, is the supercoiled F plasmid that results from the miniprep protocol.

• The next step is to perform the enzymatic digestion of the extracted F plasmid with the HindIII enzyme.

Gabriel

finO and finP's cultures miniprep

• We continued our work with finO and finP today by performing minipreps from yesterday's inoculated cultures, according to Protocol 2.

• We had 10 cultures for finO and 10 cultures for finP, in order to assure that at least one of them would have the inserted fragment into it's plasmid.

Marcelo