Team:UNICAMP-Brazil/Notebooks/September 28

(Difference between revisions)

Revision as of 17:16, 18 October 2009

Today we performed minipreps from yesterday's inoculated cultures, related to the biobricks already cited.
We followed Protocol 2, without modification.

Marcelo

Once we digested those biobricks yesterday, we could proceed today to the purification of the target band from an agarose gel.
We used Invitrogen's Purilink Quick Gel Extraction Kit, following the manufacturer's protocol ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Purification_of_DNA_fragments_from_agarose_gels Protocol 7), without modifications.

Marcelo

<p style=”text-align:justify;”>The E.coli didn´t grow in any of the plates again. =[ We discovered that the electrocompetent E.coli that we used yesterday were dead! So we transformed another electrocompetent E.coli using the rest of the ligation reactions we had. We extremely hope to find colonies tomorrow!

Raíssa and Taís

@@ Line 9: / Line 9: @@
 *<p style=”text-align:justify;”>Today we performed minipreps from yesterday's inoculated cultures, related to the biobricks already cited.</p>
 *<p style=”text-align:justify;”>We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2], without modification.</p>
+[[image:miniprep2809_17H_17J_24M.jpg]]
+[[image:miniprep2809_12O.jpg|center]]
+*<p style=”text-align:justify;”>According to both pictures, we sucessfully recovered all biobricks! :)
 ''Marcelo''
@@ Line 16: / Line 23: @@
 * Once we digested those biobricks yesterday, we could proceed today to the purification of the target band from an agarose gel.
-* We used Invitrogen's Purilink Quick Gel Extraction Kit, following the manufacturer's protocol, without modifications.
+* We used Invitrogen's Purilink Quick Gel Extraction Kit, following the manufacturer's protocol ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Purification_of_DNA_fragments_from_agarose_gels Protocol 7), without modifications.
 ''Marcelo''