Team:UNICAMP-Brazil/Notebooks/September 28

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====Minipreps from BBa_E0840, BBa_I718017, BBa_J61000 and BBa_I78016====
====Minipreps from BBa_E0840, BBa_I718017, BBa_J61000 and BBa_I78016====
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*<p style=”text-align:justify;”>Today we performed minipreps from yesterday's inoculated cultures, related to the biobricks already cited.</p>
*<p style=”text-align:justify;”>Today we performed minipreps from yesterday's inoculated cultures, related to the biobricks already cited.</p>
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[[image:miniprep2809_12O.jpg|center]]
[[image:miniprep2809_12O.jpg|center]]
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*<p style=”text-align:justify;”>According to both pictures, we sucessfully recovered all biobricks! :)
 
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*<p style=”text-align:justify;”>According to both pictures, we sucessfully recovered all biobricks! :)</p>
''Marcelo''
''Marcelo''
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* Once we digested those biobricks yesterday, we could proceed today to the purification of the target band from an agarose gel.
* Once we digested those biobricks yesterday, we could proceed today to the purification of the target band from an agarose gel.
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* We used Invitrogen's Purilink Quick Gel Extraction Kit, following the manufacturer's protocol ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Purification_of_DNA_fragments_from_agarose_gels Protocol 7), without modifications.
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* We used Invitrogen's Purilink Quick Gel Extraction Kit, following the manufacturer's protocol ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Purification_of_DNA_fragments_from_agarose_gels Protocol 7]), without modifications.
''Marcelo''
''Marcelo''

Revision as of 17:17, 18 October 2009

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ColiGuard

Minipreps from BBa_E0840, BBa_I718017, BBa_J61000 and BBa_I78016

  • Today we performed minipreps from yesterday's inoculated cultures, related to the biobricks already cited.

  • We followed Protocol 2, without modification.

Miniprep2809 17H 17J 24M.jpg

Miniprep2809 12O.jpg


  • According to both pictures, we sucessfully recovered all biobricks! :)

Marcelo


Purification of pTet and double terminator biobricks

  • Once we digested those biobricks yesterday, we could proceed today to the purification of the target band from an agarose gel.
  • We used Invitrogen's Purilink Quick Gel Extraction Kit, following the manufacturer's protocol (Protocol 7), without modifications.

Marcelo


YeastGuard

Dephosphorylation - CIAP test

  • The E.coli didn´t grow in any of the plates again. =[ We discovered that the electrocompetent E.coli that we used yesterday were dead! So we transformed another electrocompetent E.coli using the rest of the ligation reactions we had. We extremely hope to find colonies tomorrow!

Raíssa and Taís




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