Team:UNICAMP-Brazil/Notebooks/September 28

ColiGuard

Minipreps from BBa_E0840, BBa_I718017, BBa_J61000 and BBa_I78016

• Today we performed minipreps from yesterday's inoculated cultures, related to the biobricks already cited.

• We followed Protocol 2, without modification.

• According to both pictures, we sucessfully recovered all biobricks! :)

Marcelo

Purification of pTet and double terminator biobricks

• Once we digested those biobricks yesterday, we could proceed today to the purification of the target band from an agarose gel.
• We used Invitrogen's Purelink Quick Gel Extraction Kit, following the manufacturer's protocol (Protocol 7), without modifications.

Marcelo

YeastGuard

Dephosphorylation - CIAP test

• The E.coli didn´t grow in any of the plates again. =[ We discovered that the electrocompetent E.coli that we used yesterday were dead! So we transformed another electrocompetent E.coli using the rest of the ligation reactions we had. We extremely hope to find colonies tomorrow!

Raíssa and Taís