Team:UNICAMP-Brazil/Notebooks/October 9
From 2009.igem.org
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-A PCR: performed with the specific forward primer for our insert and with the reverse primer for pGEM plasmid (M13), in order to confirm that, once our inserts are indeed in there, they are also in the correct frame position. | -A PCR: performed with the specific forward primer for our insert and with the reverse primer for pGEM plasmid (M13), in order to confirm that, once our inserts are indeed in there, they are also in the correct frame position. | ||
- | * Seeing that, today we performed the digestion with | + | * Seeing that, today we performed the digestion with EcoRI and SpeI for all of our minipreps samples that actually worked. Digestion lasted 3 hours. |
[[image:Digestao0910_finO_finPempGEM.jpg]] | [[image:Digestao0910_finO_finPempGEM.jpg]] |
Revision as of 18:22, 18 October 2009
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