Team:UNICAMP-Brazil/Notebooks/October 11

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Revision as of 18:37, 18 October 2009

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ColiGuard

finOP-pGEM digestion purification

  • After confirming correctly finOP with pGEM ligation, today we gather all confirmed samples (for finO and for finP) into a single sample for each one.
  • We ran 40 uL of each gathered sample in an agarose gel for later purification. Unfortunately, finP band appeared extremely weak in the gel and we weren't able to purify it.
  • As for finO, it's band appeared in gel, but we decided to wait for finP in order to perform both purifications together.

Marcelo





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