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Team:UNICAMP-Brazil/Notebooks/October 11
From 2009.igem.org
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====finOP-pGEM digestion purification==== | ====finOP-pGEM digestion purification==== | ||
| - | * After confirming correctly finOP with pGEM | + | * After confirming correctly finOP with pGEM ligations, today we gather all confirmed samples (for finO and for finP) into a single sample for each one. |
| - | * We ran 40 uL of each gathered sample in an agarose gel for later purification. Unfortunately, finP band appeared extremely weak in the gel and we weren't able to purify it. | + | * We ran 40 uL of each gathered sample in an agarose gel for later purification. Unfortunately, finP band appeared extremely weak in the gel and we weren't able to purify it. As for finO, it's band appeared in gel, but we decided to wait for finP in order to perform both purifications together. |
| - | + | * Thus, we took all the gathered finP sample left and concentrated it in speed vacuum, until ir reaches about 20 uL. Then we ran another agarose gel with this concentrated sample and with another 40 uL from finO gathered sample. | |
| + | * This time, finP appeared as a barely visible band, but we were able to purify it, as we did for finO to. | ||
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''Marcelo'' | ''Marcelo'' | ||
Revision as of 18:49, 18 October 2009
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