Team:UNICAMP-Brazil/Notebooks/September 11

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Revision as of 18:56, 18 October 2009

We purified the PCR products PY1 and PY2 with the Invitrogen's PureLink™ PCR Purification Kit, following the manufacturer's protocol without modifications.

Fabiana and Leonardo

<p style=”text-align:justify;”>The four ligation reactions that were reacting O/N were dialyzed for one hour before undergoing transformation into E. coli (Protocol 3). 5µl of each ligation were used to transform the bacterias. After growing at 37ºC for one hour in LB medium, they were plated on LB+AMP plates and continue to grow at 37ºC O/N.

Taís

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	====New biobricks in biobrick format====		====New biobricks in biobrick format====
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	*<p style=”text-align:justify;”>The four ligation reactions that were reacting O/N were dialyzed for one hour before undergoing transformation into ''E. coli'' ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporationi Protocol 3]). 5µl of each ligation were used to transform the bacterias. After growing at 37ºC for one hour in LB medium, they were plated on LB+AMP plates and continue to grow at 37ºC O/N.</p>		*<p style=”text-align:justify;”>The four ligation reactions that were reacting O/N were dialyzed for one hour before undergoing transformation into ''E. coli'' ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporationi Protocol 3]). 5µl of each ligation were used to transform the bacterias. After growing at 37ºC for one hour in LB medium, they were plated on LB+AMP plates and continue to grow at 37ºC O/N.</p>