Team:UNICAMP-Brazil/Notebooks/July 20

(Difference between revisions)

Revision as of 19:42, 19 October 2009

During the week from July 20th to 25th we designed all primers that we´re going to use in our project. All the sequences are showed on July 25th notebook.

Everybody

After the recovery of F plasmid (July 16th), we performed a digestion of the miniprep's product, in order to assure that we indeed recover the plasmid itself. We did this assay by using two restriction enzymes: EcoRI and ClaI.
We then compared the pattern provided us by pDRAW software with an eletrophoresis gel of the digestion product. According to this assay, we indeed were able to recover the F Plasmid!

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Gabriel, Marcelo and Victor.

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 ==== Restriction Assay====
-* After the recovery of F plasmid (July 16th), we performed a digestion of the miniprep's product, in order to assure that we indeed recover the plasmid itself. We did this assay by using two restriction enzymes: ''EcoR''I and ''Cla''I. We followed protocol nº X, without modifications. We then compared the pattern provided us by pDRAW software with an eletrophoresis gel of the digestion product. According to this assay, we indeed were able to recover the F Plasmid!
+* After the recovery of F plasmid (July 16th), we performed a digestion of the miniprep's product, in order to assure that we indeed recover the plasmid itself. We did this assay by using two restriction enzymes: ''EcoR''I and ''Cla''I.
+* We then compared the pattern provided us by pDRAW software with an eletrophoresis gel of the digestion product. According to this assay, we indeed were able to recover the F Plasmid!
 =]