Team:UNICAMP-Brazil/Notebooks/September 7
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*<p style=”text-align:justify;”>In order to achieve this objective, we permorfed today the digestion with XbaI and SpeI restriction enzymes of the purified sequences finO and finP, and also of the vector pSB1A3. The digestion lasted 3 hours.</p> | *<p style=”text-align:justify;”>In order to achieve this objective, we permorfed today the digestion with XbaI and SpeI restriction enzymes of the purified sequences finO and finP, and also of the vector pSB1A3. The digestion lasted 3 hours.</p> | ||
*<p style=”text-align:justify;”>After that, we run an agarose gel of the digested product aiming the confirmation of the assay. According to the gel, the vector pSB1A3 was succesful digested. As for the sequences finO and finP, it's not possible to assure whether they were digested or not since the gel resolution does not differentiate just a few base pairs (excised by digestion). Therefore, we need to ligate those sequences into the digested vector in order to confirm that they both were also digested.</p> | *<p style=”text-align:justify;”>After that, we run an agarose gel of the digested product aiming the confirmation of the assay. According to the gel, the vector pSB1A3 was succesful digested. As for the sequences finO and finP, it's not possible to assure whether they were digested or not since the gel resolution does not differentiate just a few base pairs (excised by digestion). Therefore, we need to ligate those sequences into the digested vector in order to confirm that they both were also digested.</p> | ||
- | *<p style=”text-align:justify;”>FinO appeared in the gel, altough not very visile. As for finP, no visible band was found. We | + | *<p style=”text-align:justify;”>FinO appeared in the gel, altough not very visile. As for finP, no visible band was found. We thought that this was kind of expected, since finP's band was very weak even before the digestion procedure, and we didn't ran enough volume. |
[[image:IGEM_digest_finOfinPVetorKd_07092009.jpg|center]] | [[image:IGEM_digest_finOfinPVetorKd_07092009.jpg|center]] |
Revision as of 21:04, 19 October 2009
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