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Team:Newcastle/Labwork/28 September 2009
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Previously Metal Sensing Team tried to ligate cotC biobrick with pmutin4 and transform E.coli DH5 cells. Although the transformations have worked, the control plates wothout the insert had also colonies. | Previously Metal Sensing Team tried to ligate cotC biobrick with pmutin4 and transform E.coli DH5 cells. Although the transformations have worked, the control plates wothout the insert had also colonies. | ||
Revision as of 08:43, 20 October 2009
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Formal Lab Session - 28th September 2009
Previously Metal Sensing Team tried to ligate cotC biobrick with pmutin4 and transform E.coli DH5 cells. Although the transformations have worked, the control plates wothout the insert had also colonies.
We selected 10 colonies from the plate with the insert + the backbone and inoculated to LB+Amp for ON cultures for tomorrow's miniprep.
We prepared some agar gel.
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News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
