Team:UNICAMP-Brazil/Notebooks/August 11

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==''' YeastGuard '''==
==''' YeastGuard '''==
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====Recovering the biobricks====
We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions.
We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions.

Revision as of 01:50, 21 October 2009

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ColiGuard

Selection of transformants

Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetaly in LB medium containing the appropriate antibiotic and let them grow O/N.

YeastGuard

Recovering the biobricks

We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions.

As we need to get the DNA of BBa_J63002, we decided to repeat the plasmid extractions of the other five biobricks we are really going to use: BBa_J63005, BBa_J63003, BBa_E2030, BBa_E2020 and BBa_J63010.

In order to do the miniprep tomorrow, we selected the colonies of each transformation (of the six biobricks) and innoculated them in LB medium containing the appropriate antibiotic to grow O/N.

Taís




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