Team:UNICAMP-Brazil/Notebooks/August 20

From 2009.igem.org

(Difference between revisions)
(→YeastGuard)
Line 6: Line 6:
==''' YeastGuard '''==
==''' YeastGuard '''==
-
==== Terminator biobrick ====
+
====Recovering the biobricks====
-
The colonies didn’t grow in the liquid LB medium.
+
-
==== RBS biobrick ====
+
* The terminator colonies didn’t grow in the liquid LB medium.
-
We performed miniprep to get purified RBS biobrick ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]).  
+
 
 +
* We performed miniprep to get purified RBS biobrick ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]).
 +
 
 +
''Taís''
==''' ColiGuard '''==
==''' ColiGuard '''==

Revision as of 02:11, 21 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

YeastGuard

Recovering the biobricks

  • The terminator colonies didn’t grow in the liquid LB medium.
  • We performed miniprep to get purified RBS biobrick (Protocol 2).

Taís

ColiGuard

Lux S (BBa_K091109) enzyme restriction

We tried one more time the enzyme restriction with XbaI and PstI, but didn’t find the part again. We expected 530 band size, but just was noticed plasmid band. Something is wrong with this part.

‎

Luige




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/August_20"