Team:UNICAMP-Brazil/Notebooks/October 14

From 2009.igem.org

(Difference between revisions)
Tsherig (Talk | contribs)
(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== ====New strategy: pGEM==== *<p style=”text-align:justify;”>We did miniprep...)
Newer edit →

Revision as of 03:14, 21 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

YeastGuard

New strategy: pGEM

  • We did miniprep of 10 Adh1+Lysozyme colonies.

  • We digested the Adh1+Lysozyme/biofusion minipreps in order to connect it to the yeast expression vector (YEP). We used the XbaI and PstI enzymes. 9 of 10 digestions worked! =) The expected fragment size is 2000bp.

GEL

  • We chose two fragments and purified them from the agarose gel. We ligated them to YEP. We will transform it tomorrow.


Raíssa and Taís




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_14"