Team:UNICAMP-Brazil/Notebooks/August 28
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* Colony PCR | * Colony PCR | ||
The colony PCR was made to confirm the presence of terminator transformants, because the antibiotic resistence is not working to any antibiotics. | The colony PCR was made to confirm the presence of terminator transformants, because the antibiotic resistence is not working to any antibiotics. | ||
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+ | [[Image:PCRKL.jpg|350px|center]] | ||
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We plated the cells in LB and picked up some colonies with a toothpick, inoculated on a backup LB plate and put the toothpick on a tube with 20µl H2O, then, denatured this cells 95°C, 5 minutes and heat shock on ice 5 minutes. Spin the tubes and use 1µl to make the PCR (see protocol). | We plated the cells in LB and picked up some colonies with a toothpick, inoculated on a backup LB plate and put the toothpick on a tube with 20µl H2O, then, denatured this cells 95°C, 5 minutes and heat shock on ice 5 minutes. Spin the tubes and use 1µl to make the PCR (see protocol). |
Revision as of 03:14, 21 October 2009
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