Team:UNICAMP-Brazil/Notebooks/October 19

From 2009.igem.org

(Difference between revisions)
Tsherig (Talk | contribs)
(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== ====New strategy: pGEM==== *<p style=”text-align:justify;”>We sent the pDL...)
Newer edit →

Revision as of 03:56, 21 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

YeastGuard

New strategy: pGEM

  • We sent the pDLD+lysozyme device to iGEM today. =)

Yeast experiments

  • There are plenty of yeast colonies in the pDLD+Lys and Adh1+Lys plates. =)

  • We did PCR with 30 yeast colonies transformed with Adh1+Lysozyme and pDLD+Lysozyme. Unfortunately none of the colonies had the correct vector =/

GEL GEL

  • We have no time to repeat the transformations =( Our hope is that there has been a problem to the PCR reaction and that the parts are correctly connected to the YEP vector. So we decided to go on with the transformed yeasts we have.

  • At night we find plenty yeast colonies in the pDLD+YFP and Adh1+YFP plates.

  • Considering our lack of time we decided to collect a pool of yeast colonies for each construction and inoculate in liquid media. This strategy was adopted for both constructions (pDLD+YFP and Adh1+YFP). These pre inocula will be used for induction tests of Lysozyme tomorrow.


Raíssa and Taís




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_19"