Team:UNICAMP-Brazil/Notebooks/August 11

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Revision as of 05:45, 21 October 2009

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ColiGuard

Selection of transformants

Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetaly in LB medium containing the appropriate antibiotic and let them grow O/N.

YeastGuard

Recovering the biobricks

We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions.

We will need large amounts of DNA so we inoculated colonies in liquid media in order to repeated the plasmid extractions of the six biobricks we are really going to use.

Taís

@@ Line 13: / Line 13: @@
 ====Recovering the biobricks====
-We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions.
+* We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions.
-As we need to get the DNA of BBa_J63002, we decided to repeat the plasmid extractions of the other five biobricks we are really going to use: BBa_J63005, BBa_J63003, BBa_E2030, BBa_E2020 and BBa_J63010.
+* We will need large amounts of DNA so we inoculated colonies in liquid media in order to repeated the plasmid extractions of the six biobricks we are really going to use.
-In order to do the miniprep tomorrow, we selected the colonies of each transformation (of the six biobricks) and innoculated them in LB medium containing the appropriate antibiotic to grow O/N.
 ''Taís''
 {{:Team:UNICAMP-Brazil/inc_rodape}}