Team:Kyoto/CiC/Notebook/0907-0911
From 2009.igem.org
(Difference between revisions)
(→0914-0918 : what to do. what to do. what to do. what to do.) |
(→0914-0918 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator) |
||
Line 29: | Line 29: | ||
</html> | </html> | ||
{{:Team:Kyoto/CiC/Notebook_tpl}} | {{:Team:Kyoto/CiC/Notebook_tpl}} | ||
- | == | + | ==097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator == |
{{:Team:Kyoto/Pad_start_dates}} | {{:Team:Kyoto/Pad_start_dates}} | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
===Wednesday, 16 September=== | ===Wednesday, 16 September=== | ||
====To Do==== | ====To Do==== | ||
- | ** | + | *PCR |
- | ** | + | **HIV-TAT-(LALAAAA)3 |
- | + | *Restriction Enzyme Digestion | |
+ | **HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1) | ||
+ | **B0015 (EcoR1 + Spe1) | ||
+ | *Electrophoresis | ||
+ | **HIV-TAT-(LALAAAA)3 | ||
+ | **B0015 | ||
====Results==== | ====Results==== | ||
=====ligation===== | =====ligation===== |
Revision as of 09:44, 21 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator
Wednesday, 16 September
To Do
- PCR
- HIV-TAT-(LALAAAA)3
- Restriction Enzyme Digestion
- HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1)
- B0015 (EcoR1 + Spe1)
- Electrophoresis
- HIV-TAT-(LALAAAA)3
- B0015
Results
ligation
NO. | Sample name | conc./(ng/ul) |
---|---|---|
1 | 11(stu1) | 103.2 |
Transformation
No colony was observed (090917 10:00)
Thursday, 17 September
To Do
- Make repetitive sequence (MPR)
- Transformation(retry)