Team:Kyoto/CiC/Notebook/0907-0911

From 2009.igem.org

(Difference between revisions)
(→ligation)
(→Results)
Line 54: Line 54:
!NO.!!Sample name!!conc./(ng/ul)
!NO.!!Sample name!!conc./(ng/ul)
|-
|-
-
|1||HIV-TAT-(LALAAAA)3(EcoR1,Spe1)sample1||36.2
+
|1||HIV-TAT-(LALAAAA)3 sample1(EcoR1,Spe1)||36.2
|-
|-
-
|2||HIV-TAT-(LALAAAA)3(EcoR1,Spe1)sample2||21.7
+
|2||HIV-TAT-(LALAAAA)3 sample2(EcoR1,Spe1)||21.7
|-
|-
-
|3||HIV-TAT-(LALAAAA)3(EcoR1,Spe1)sample3||47.9
+
|3||HIV-TAT-(LALAAAA)3 sample3(EcoR1,Spe1)||47.9
|-
|-
|4||B0015(EcoR1,Xba1)||2.48
|4||B0015(EcoR1,Xba1)||2.48

Revision as of 09:59, 21 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator

Wednesday, 16 September

To Do

  • PCR
    • HIV-TAT-(LALAAAA)3
  • Restriction Enzyme Digestion
    • HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1)
    • B0015 (EcoR1 + Spe1)
  • Electrophoresis

Results

Fryday, 11 September

To Do

  • Ligation
  • Transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
1HIV-TAT-(LALAAAA)3 sample1(EcoR1,Spe1)36.2
2HIV-TAT-(LALAAAA)3 sample2(EcoR1,Spe1)21.7
3HIV-TAT-(LALAAAA)3 sample3(EcoR1,Spe1)47.9
4B0015(EcoR1,Xba1)2.48
Transformation

No colony was observed (090917 10:00)　

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/0907-0911"