Team:Kyoto/CiC/Notebook/0914-0918

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===Tuesday, 15 September===
===Tuesday, 15 September===
====To Do====
====To Do====
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'''Further to 14 Sept.'''
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*知らん
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{{:Team:Kyoto/Pad_between_dates}}
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*p-2-(1)(4)(7)(8)(10)
 
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**Miniprep
 
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**Restriction Enzyme Digestion
 
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*p-2-(1)(4)  E.X
 
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**PCR purification kit
 
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*p-2-(1)(7)(8)(10)  E.S
 
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**Gel extraction
 
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*4-(1)(2)(3)
 
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**Colony PCR
 
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**Insert check
 
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{{:Team:Kyoto/Pad_between_dates}}
 
===Wednesday, 16 September===
===Wednesday, 16 September===
====To Do====
====To Do====

Revision as of 10:09, 21 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : what to do. what to do. what to do. what to do.

Monday, 14 September

To Do

  • わからん

Results

Tuesday, 15 September

To Do

  • 知らん

Wednesday, 16 September

To Do

    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
111(stu1)103.2
Transformation

No colony was observed (090917 10:00)　

Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/0914-0918"