Team:UNICAMP-Brazil/Notebooks/September 30
From 2009.igem.org
Line 47: | Line 47: | ||
====Digestion of Biobricks BBa_E0840, BBa_I718017, BBa_J61000 and BBa_I718016==== | ====Digestion of Biobricks BBa_E0840, BBa_I718017, BBa_J61000 and BBa_I718016==== | ||
- | Today, we made miniprep ([Team:UNICAMP-Brazil/Protocols/Mini-Prep protocol 2]) of the selected colonies yesterday. After, we performed two PCR. The first reaction with VF2 and VR primer and the second reaction with Anticol F and Anticol R primer (designed by us) in order to prove the correct transformation. Lamentably, we didn’t get it. In addition we made also a digestion reaction with EcoRI and PstI. We didn’t see the band size expected. Definitively, the CeiB isn’t into the plasmid. We decide to try searching. Otherwise we repeated the cell transformation with CeaB DNA, we made colony PCR but don’t see the right size band. | + | Today, we made miniprep ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep protocol 2]) of the selected colonies yesterday. After, we performed two PCR. The first reaction with VF2 and VR primer and the second reaction with Anticol F and Anticol R primer (designed by us) in order to prove the correct transformation. Lamentably, we didn’t get it. In addition we made also a digestion reaction with EcoRI and PstI. We didn’t see the band size expected. Definitively, the CeiB isn’t into the plasmid. We decide to try searching. Otherwise we repeated the cell transformation with CeaB DNA, we made colony PCR but don’t see the right size band. |
''Luige'' | ''Luige'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} |
Revision as of 16:09, 21 October 2009
|