Deposited BioBricks.html

From 2009.igem.org

(Difference between revisions)
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{| border="1"
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{{SLOtemp|
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!Name
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Content=
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!Type
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__NOTOC__
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!Description
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-
!Length
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=Designed BioBricks=
-
|-
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We have designed four NEW functionalised linker-extension standard vectors. All together, 106 parts were generated all cloned into our NEW functionalised linker-extension standard vectors and forty-one were single coding, thirteen intermediates and forty-eight composite parts.  
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245005 BBa_K245005]
+
 
-
|Plasmid_Backbone
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==Plasmid backbones==
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|BB-NIC-II-HisN
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Four NEW linker-extension standard vectors are constructed.
-
|2261
+
 
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245006 BBa_K245006]
+
-
|Plasmid_Backbone
+
-
|BB-NIC-II-NC
+
-
|2240
+
-
|-
+
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]
+
-
|Plasmid_Backbone
+
-
|BB-NIC-II-HisC
+
-
|2167
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]
+
-
|Plasmid_Backbone
+
-
|BB-NIC-III-HisN
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-
|2261
+
-
|}
+
<br>
<br>
 +
 +
<table class="crte">
 +
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245005 BBa_K245005]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-HisN</td><td>2261</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245006 BBa_K245006]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-NC</td><td>2240</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-HisC</td><td>2167</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]</td><td>Plasmid_Backbone</td><td>BB-NIC-III-HisN</td><td>2261</td></tr>
 +
</table>
 +
<br>
<br>
-
{| border="1"
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!Name
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==The coding sequences==
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!Type
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The forty-one BioBrick coding sequences are prepared and are listed as (i) coiled-coil-forming segments, (ii) oligimerisation domains, (iii) functional domains and others.  
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!Description
+
-
!Length
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245038 BBa_K245038]
+
-
|Coding
+
-
|NZ - cc homodimer
+
-
|87
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245039 BBa_K245039]
+
-
|Coding
+
-
|CZ
+
-
|90
+
-
|-
+
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245118 BBa_K245118]
+
-
|Coding
+
-
|P1
+
-
|99
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245119 BBa_K245119]
+
-
|Coding
+
-
|P2
+
-
|99
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245120 BBa_K245120]
+
-
|Coding
+
-
|P3
+
-
|99
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245121 BBa_K245121]
+
-
|Coding
+
-
|P4
+
-
|99
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245122 BBa_K245122]
+
-
|Coding
+
-
|P5
+
-
|99
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245123 BBa_K245123]
+
-
|Coding
+
-
|P6
+
-
|99
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245124 BBa_K245124]
+
-
|Coding
+
-
|P7
+
-
|99
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245125 BBa_K245125]
+
-
|Coding
+
-
|P8
+
-
|99
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245126 BBa_K245126]
+
-
|Coding
+
-
|H1
+
-
|99
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245127 BBa_K245127]
+
-
|Coding
+
-
|GCN
+
-
|99
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245132 BBa_K245132]
+
-
|Coding
+
-
|APH1
+
-
|90
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245133 BBa_K245133]
+
-
|Coding
+
-
|APH
+
-
|135
+
-
|-
+
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245134 BBa_K245134]
+
-
|Coding
+
-
|BCR
+
-
|108
+
-
|}
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<br>
<br>
<br>
<br>
-
{| border="1"
+
===Coiled-coil segments===
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!Name
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-
!Type
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-
!Description
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-
!Length
+
-
|-
+
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245026 BBa_K245026]
+
-
|Coding
+
-
|TA
+
-
|63
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245027 BBa_K245027]
+
-
|Coding
+
-
|TB
+
-
|63
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245028 BBa_K245028]
+
-
|Coding
+
-
|TC
+
-
|63
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245029 BBa_K245029]
+
-
|Coding
+
-
|T1W_DDD
+
-
|189
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245030 BBa_K245030]
+
-
|Coding
+
-
|T1W_KKK
+
-
|189
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245031 BBa_K245031]
+
-
|Coding
+
-
|T1W_EEE
+
-
|186
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245042 BBa_K245042]
+
-
|Coding
+
-
|VASP
+
-
|135
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245116 BBa_K245116]
+
-
|Coding
+
-
|GyrB
+
-
|657
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245117 BBa_K245117]
+
-
|Coding
+
-
|GyrB-SG
+
-
|663
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245128 BBa_K245128]
+
-
|Coding
+
-
|p53
+
-
|90
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245129 BBa_K245129]
+
-
|Coding
+
-
|CutA1
+
-
|333
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245130 BBa_K245130]
+
-
|Coding
+
-
|T1W_AEA
+
-
|186
+
-
|-
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245141 BBa_K245141]
+
-
|Coding
+
-
|foldon
+
-
|81
+
-
|}
+
<br>
<br>
 +
<table class="crte">
 +
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245038 BBa_K245038]</td><td>Coding</td><td>NZ - cc homodimer</td><td>87</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245039 BBa_K245039]</td><td>Coding</td><td>CZ</td><td>90</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245118 BBa_K245118]</td><td>Coding</td><td>P1</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245119 BBa_K245119]</td><td>Coding</td><td>P2</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245120 BBa_K245120]</td><td>Coding</td><td>P3</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245121 BBa_K245121]</td><td>Coding</td><td>P4</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245122 BBa_K245122]</td><td>Coding</td><td>P5</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245123 BBa_K245123]</td><td>Coding</td><td>P6</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245124 BBa_K245124]</td><td>Coding</td><td>P7</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245125 BBa_K245125]</td><td>Coding</td><td>P8</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245126 BBa_K245126]</td><td>Coding</td><td>H1</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245127 BBa_K245127]</td><td>Coding</td><td>GCN</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245132 BBa_K245132]</td><td>Coding</td><td>APH1</td><td>90</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245133 BBa_K245133]</td><td>Coding</td><td>APH</td><td>135</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245134 BBa_K245134]</td><td>Coding</td><td>BCR</td><td>108</td></tr>
 +
</table>
 +
<br>
<br>
-
{| border="1"
 
-
!Name
 
-
!Type
 
-
!Description
 
-
!Length
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]
 
-
|Coding
 
-
|cYFP
 
-
|252
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]
 
-
|Coding
 
-
|nYFP
 
-
|462
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245024 BBa_K245024]
 
-
|Coding
 
-
|Z1
 
-
|36
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245025 BBa_K245025]
 
-
|Coding
 
-
|AUP
 
-
|36
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245046 BBa_K245046]
 
-
|Coding
 
-
|ccdB
 
-
|306
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245113 BBa_K245113]
 
-
|Coding
 
-
|KSI-DP
 
-
|378
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114]
 
-
|Coding
 
-
|LL37
 
-
|111
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]
 
-
|Coding
 
-
|KSI-DP-P2/33
 
-
|405
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245140 BBa_K245140]
 
-
|Coding
 
-
|mNGF
 
-
|669
 
-
|}
 
<br>
<br>
 +
===Oligomerization domains===
 +
<table class="crte">
 +
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245026 BBa_K245026]</td><td>Coding</td><td>TA</td><td>63</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245027 BBa_K245027]</td><td>Coding</td><td>TB</td><td>63</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245028 BBa_K245028]</td><td>Coding</td><td>TC</td><td>63</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245029 BBa_K245029]</td><td>Coding</td><td>T1W_DDD</td><td>189</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245030 BBa_K245030]</td><td>Coding</td><td>T1W_KKK</td><td>189</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245031 BBa_K245031]</td><td>Coding</td><td>T1W_EEE</td><td>186</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245042 BBa_K245042]</td><td>Coding</td><td>VASP</td><td>135</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245116 BBa_K245116]</td><td>Coding</td><td>GyrB</td><td>657</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245117 BBa_K245117]</td><td>Coding</td><td>GyrB-SG</td><td>663</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245128 BBa_K245128]</td><td>Coding</td><td>p53</td><td>90</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245129 BBa_K245129]</td><td>Coding</td><td>CutA1</td><td>333</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245130 BBa_K245130]</td><td>Coding</td><td>T1W_AEA</td><td>186</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245141 BBa_K245141]</td><td>Coding</td><td>foldon</td><td>81</td></tr>
 +
</table>
 +
<br>
<br>
-
{| border="1"
 
-
!Name
 
-
!Type
 
-
!Description
 
-
!Length
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245131 BBa_K245131]
 
-
|Coding
 
-
|ELST
 
-
|150
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245139 BBa_K245139]
 
-
|Coding
 
-
|RADA
 
-
|66
 
-
|}
 
<br>
<br>
 +
 +
===Functional domains===
 +
<table class="crte">
 +
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]</td><td>Coding</td><td>cYFP</td><td>252</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]</td><td>Coding</td><td>nYFP</td><td>462</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245024 BBa_K245024]</td><td>Coding</td><td>Z1</td><td>36</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245025 BBa_K245025]</td><td>Coding</td><td>AUP</td><td>36</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245046 BBa_K245046]</td><td>Coding</td><td>ccdB</td><td>306</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245113 BBa_K245113]</td><td>Coding</td><td>KSI-DP</td><td>378</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114]</td><td>Coding</td><td>LL37</td><td>111</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]</td><td>Coding</td><td>KSI-DP-P2/33</td><td>405</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245140 BBa_K245140]</td><td>Coding</td><td>mNGF</td><td>669</td></tr>
 +
</table>
 +
<br>
<br>
-
{| border="1"
 
-
!Name
 
-
!Type
 
-
!Description
 
-
!Length
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245048 BBa_K245048]
 
-
|Intermediate
 
-
|H1-P2
 
-
|204
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245049 BBa_K245049]
 
-
|Intermediate
 
-
|KSI-DP-P1
 
-
|489
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245052 BBa_K245052]
 
-
|Intermediate
 
-
|GCN_P2
 
-
|204
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245053 BBa_K245053]
 
-
|Intermediate
 
-
|P4-H1
 
-
|204
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245054 BBa_K245054]
 
-
|Intermediate
 
-
|P2-H1
 
-
|204
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245055 BBa_K245055]
 
-
|Intermediate
 
-
|P6-H1
 
-
|204
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245056 BBa_K245056]
 
-
|Intermediate
 
-
|P3-P5
 
-
|204
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245057 BBa_K245057]
 
-
|Intermediate
 
-
|P4-P2
 
-
|204
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245059 BBa_K245059]
 
-
|Intermediate
 
-
|LL37-ELST
 
-
|267
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245060 BBa_K245060]
 
-
|Intermediate
 
-
|ELST-ELST-ELST
 
-
|462
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245061 BBa_K245061]
 
-
|Intermediate
 
-
|ELST-ELST
 
-
|306
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245096 BBa_K245096]
 
-
|Intermediate
 
-
|BCR-P2
 
-
|213
 
-
|-
 
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245097 BBa_K245097]
 
-
|Intermediate
 
-
|APH-P2
 
-
|240
 
-
|}
 
<br>
<br>
 +
 +
===Others===
 +
<table class="crte">
 +
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245131 BBa_K245131]</td><td>Coding</td><td>ELST</td><td>150</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245139 BBa_K245139]</td><td>Coding</td><td>RADA</td><td>66</td></tr>
 +
</table>
 +
<br>
<br>
 +
<br>
 +
 +
==The intermediate sequences==
 +
Thirteen BioBricks with intermediate parts are constructed.
 +
<br>
 +
<table class="crte">
 +
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245048 BBa_K245048]</td><td>Intermediate</td><td>H1-P2</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245049 BBa_K245049]</td><td>Intermediate</td><td>KSI-DP-P1</td><td>489</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245052 BBa_K245052]</td><td>Intermediate</td><td>GCN_P2</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245053 BBa_K245053]</td><td>Intermediate</td><td>P4-H1</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245054 BBa_K245054]</td><td>Intermediate</td><td>P2-H1</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245055 BBa_K245055]</td><td>Intermediate</td><td>P6-H1</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245056 BBa_K245056]</td><td>Intermediate</td><td>P3-P5</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245057 BBa_K245057]</td><td>Intermediate</td><td>P4-P2</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245059 BBa_K245059]</td><td>Intermediate</td><td>LL37-ELST</td><td>267</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245060 BBa_K245060]</td><td>Intermediate</td><td>ELST-ELST-ELST</td><td>462</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245061 BBa_K245061]</td><td>Intermediate</td><td>ELST-ELST</td><td>306</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245096 BBa_K245096]</td><td>Intermediate</td><td>BCR-P2</td><td>213</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245097 BBa_K245097]</td><td>Intermediate</td><td>APH-P2</td><td>240</td></tr>
 +
</table>
 +
 +
<br>
 +
<br>
 +
 +
==Composite parts==
 +
The forty-eight BioBricks as composites are prepared, which are used in (i) polypeptide manufacturing, (ii) polypeptide membranes, (iii) coiled-coil polyhedra, (iv) regulated assembly and (v) functional biopolymers.
 +
 +
<br>
 +
<br>
 +
 +
===Polypeptide manufacturing===
 +
<table class="crte">
 +
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]</td><td>Coding</td><td>KSI-DP-P2/33</td><td>405</td></tr>
 +
 +
 +
<br>
 +
<br>
 +
 +
===Polypeptide membrane===
 +
<table class="crte">
 +
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245091 BBa_K245091]</td><td>Composite</td><td>p53-APH1</td><td>186</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245092 BBa_K245092]</td><td>Composite</td><td>p53-APH</td><td>231</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245093 BBa_K245093]</td><td>Composite</td><td>p53-BCR</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245099 BBa_K245099]</td><td>Composite</td><td>APH-p53</td><td>231</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245100 BBa_K245100]</td><td>Composite</td><td>BCR-p53</td><td>204</td></tr>
 +
</table>
 +
 +
<br>
 +
<br>
 +
 +
===Coiled-coil polyhedra===
 +
<table class="crte">
 +
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245066 BBa_K245066]</td><td>Composite</td><td>P1-GCN-P2</td><td>309</td></tr>
 +
</table>
 +
 +
<br>
 +
<br>
 +
 +
===Regulated assembly===
 +
<table class="crte">
 +
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245098 BBa_K245098]</td><td>Composite</td><td>GyrB-foldon</td><td>744</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245050 BBa_K245050]</td><td>Composite</td><td>GyrB-CutA1</td><td>996</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245051 BBa_K245051]</td><td>Composite</td><td>GyrB-SG-SG-CutA1</td><td>1002</td></tr>
 +
</table>
 +
 +
<br>
 +
<br>
 +
 +
===Functional biopolymers===
 +
<table class="crte">
 +
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245102 BBa_K245102]</td><td>Composite</td><td>LL37-ELST-GCN-ELST-P2</td><td>633</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245105 BBa_K245105]</td><td>Composite</td><td>ELST-ELST-ELST-mNGF</td><td>1137</td></tr>
 +
</table>
 +
 +
<br>
 +
<br>
 +
=Slovenia 2009 iGEM Team Parts Sandbox=
 +
<br>
 +
<br>
 +
<table class="crte">
 +
<tr><td><b>Name</b></td><td><b>Type</b></td><td><b>Description</b></td><td><b>Length</b></td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245005 BBa_K245005]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-HisN</td><td>2261</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245006 BBa_K245006]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-NC</td><td>2240</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-HisC</td><td>2167</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]</td><td>Plasmid_Backbone</td><td>BB-NIC-III-HisN</td><td>2261</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245009 BBa_K245009]</td><td>DNA</td><td>RFC 37 Scar Sequence</td><td>6</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]</td><td>Coding</td><td>cYFP</td><td>252</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]</td><td>Coding</td><td>nYFP</td><td>462 </td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245024 BBa_K245024]</td><td>Coding</td><td>Z1</td><td>36</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245025 BBa_K245025]</td><td>Coding</td><td>AUP</td><td>36</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245026 BBa_K245026]</td><td>Coding</td><td>TA</td><td>63</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245027 BBa_K245027]</td><td>Coding</td><td>TB</td><td>63</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245028 BBa_K245028]</td><td>Coding</td><td>TC</td><td>63</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245029 BBa_K245029]</td><td>Coding</td><td>T1W_DDD</td><td>189</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245030 BBa_K245030]</td><td>Coding</td><td>T1W_KKK</td><td>189</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245031 BBa_K245031]</td><td>Coding</td><td>T1W_EEE</td><td>186</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245038 BBa_K245038]</td><td>Coding</td><td>NZ - cc homodimer</td><td>87</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245039 BBa_K245039]</td><td>Coding</td><td>CZ</td><td>90</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245042 BBa_K245042]</td><td>Coding</td><td>VASP</td><td>135</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245046 BBa_K245046]</td><td>Coding</td><td>ccdB</td><td>306</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245048 BBa_K245048]</td><td>Intermediate</td><td>H1-P2</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245049 BBa_K245049]</td><td>Intermediate</td><td>KSI-DP-P1</td><td>489</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245050 BBa_K245050]</td><td>Composite</td><td>GyrB-CutA1</td><td>996</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245051 BBa_K245051]</td><td>Composite</td><td>GyrB-SG-SG-CutA1</td><td>1002</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245052 BBa_K245052]</td><td>Intermediate</td><td>GCN_P2</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245053 BBa_K245053]</td><td>Intermediate</td><td>P4-H1</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245054 BBa_K245054]</td><td>Intermediate</td><td>P2-H1</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245055 BBa_K245055]</td><td>Intermediate</td><td>P6-H1</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245056 BBa_K245056]</td><td>Intermediate</td><td>P3-P5</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245057 BBa_K245057]</td><td>Intermediate</td><td>P4-P2</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245058 BBa_K245058]</td><td>Composite</td><td>KSI-DP-P6</td><td>489</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245059 BBa_K245059]</td><td>Intermediate</td><td>LL37-ELST</td><td>267</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245060 BBa_K245060]</td><td>Intermediate</td><td>ELST-ELST-ELST</td><td>462</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245061 BBa_K245061]</td><td>Intermediate</td><td>ELST-ELST</td><td>306</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245062 BBa_K245062]</td><td>Composite</td><td>P1-H1-P2</td><td>309</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245063 BBa_K245063]</td><td>Composite</td><td>KSI-DP-P1-H1-P2</td><td>699</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245064 BBa_K245064]</td><td>Composite</td><td>P1-BCR-P2</td><td>318</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245065 BBa_K245065]</td><td>Composite</td><td>P1-APH-P2</td><td>345</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245066 BBa_K245066]</td><td>Composite</td><td>P1-GCN-P2</td><td>309</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245067 BBa_K245067]</td><td>Composite</td><td>P3-P4-H1</td><td>309</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245068 BBa_K245068]</td><td>Composite</td><td>P1-P3-P5</td><td>309</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245069 BBa_K245069]</td><td>Composite</td><td>KSI-DP-P1-P3-P5</td><td>699</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245070 BBa_K245070]</td><td>Composite</td><td>P6-P4-P2</td><td>309</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245071 BBa_K245071]</td><td>Composite</td><td>KSI-DP-P6-P4-P2</td><td>699</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245072 BBa_K245072]</td><td>Composite</td><td>KSI-DP-LL37-ELST</td><td>657</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245073 BBa_K245073]</td><td>Composite</td><td>KSI-DP-LL37-ELST-ELST-ELST-ELST</td><td>1125</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245074 BBa_K245074]</td><td>Composite</td><td>KSI-DP-LL-37-ELST-GCN-ELST</td><td>918</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245075 BBa_K245075]</td><td>Composite</td><td>KSI-DP-EEE</td><td>576</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245076 BBa_K245076]</td><td>Composite</td><td>KSI_DP-LL37-ELST-P2-ELST</td><td>918</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245077 BBa_K245077]</td><td>Composite</td><td>KSI-DP-LL37-ELST-P4-ELST</td><td>918</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245078 BBa_K245078]</td><td>Composite</td><td>LL37-ELST-GCN-ELST</td><td>528</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245079 BBa_K245079]</td><td>Composite</td><td>GCN-ELST</td><td>255</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245080 BBa_K245080]</td><td>DNA</td><td>scar Xba/Spe NgoMIV</td><td>12</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245081 BBa_K245081]</td><td>Composite</td><td>P2-ELST</td><td>255</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245082 BBa_K245082]</td><td>Composite</td><td>P4-ELST</td><td>255</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245083 BBa_K245083]</td><td>Composite</td><td>TA-P1</td><td>168</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245084 BBa_K245084]</td><td>Composite</td><td>TB-P3</td><td>168</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245085 BBa_K245085]</td><td>Composite</td><td>TC-P5</td><td>168</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245086 BBa_K245086]</td><td>Composite</td><td>P2-TA</td><td>168</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245087 BBa_K245087]</td><td>Composite</td><td>P4-TB</td><td>168</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245088 BBa_K245088]</td><td>Composite</td><td>TC-P5</td><td>168</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245089 BBa_K245089]</td><td>Composite</td><td>foldon-APH</td><td>222</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245090 BBa_K245090]</td><td>Composite</td><td>foldon-BCR</td><td>195</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245091 BBa_K245091]</td><td>Composite</td><td>p53-APH1</td><td>186</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245092 BBa_K245092]</td><td>Composite</td><td>p53-APH</td><td>231</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245093 BBa_K245093]</td><td>Composite</td><td>p53-BCR</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245094 BBa_K245094]</td><td>Composite</td><td>foldon-p53</td><td>177</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245095 BBa_K245095]</td><td>Composite</td><td>GyrB-p53</td><td>753</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245096 BBa_K245096]</td><td>Intermediate</td><td>BCR-P2</td><td>213</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245097 BBa_K245097]</td><td>Intermediate</td><td>APH-P2</td><td>240</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245098 BBa_K245098]</td><td>Composite</td><td>GyrB-foldon</td><td>744</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245099 BBa_K245099]</td><td>Composite</td><td>APH-p53</td><td>231</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245100 BBa_K245100]</td><td>Composite</td><td>BCR-p53</td><td>204</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245101 BBa_K245101]</td><td>Composite</td><td>LL37-ELST-GCN-ELST-P1</td><td>633</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245102 BBa_K245102]</td><td>Composite</td><td>LL37-ELST-GCN-ELST-P2</td><td>633</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245105 BBa_K245105]</td><td>Composite</td><td>ELST-ELST-ELST-mNGF</td><td>1137</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245106 BBa_K245106]</td><td>Composite</td><td>RADA-mNGF</td><td>741</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245107 BBa_K245107]</td><td>Composite</td><td>LL37-ELST-GCN-ELST-mNGF</td><td>1203</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245108 BBa_K245108]</td><td>Composite</td><td>KSI-DP-P2/33-LL37-ELST-GCN-ELST</td><td>945</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245111 BBa_K245111]</td><td>Composite</td><td>P2-P6-H1</td><td>309</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245112 BBa_K245112]</td><td>Composite</td><td>P5-P2-H1</td><td>309</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245113 BBa_K245113]</td><td>Coding</td><td>KSI-DP</td><td>378</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114]</td><td>Coding</td><td>LL37</td><td>111</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]</td><td>Coding</td><td>KSI-DP-P2/33</td><td>405</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245116 BBa_K245116]</td><td>Coding</td><td>GyrB</td><td>657</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245117 BBa_K245117]</td><td>Coding</td><td>GyrB-SG</td><td>663</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245118 BBa_K245118]</td><td>Coding</td><td>P1</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245119 BBa_K245119]</td><td>Coding</td><td>P2</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245120 BBa_K245120]</td><td>Coding</td><td>P3</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245121 BBa_K245121]</td><td>Coding</td><td>P4</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245122 BBa_K245122]</td><td>Coding</td><td>P5</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245123 BBa_K245123]</td><td>Coding</td><td>P6</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245124 BBa_K245124]</td><td>Coding</td><td>P7</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245125 BBa_K245125]</td><td>Coding</td><td>P8</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245126 BBa_K245126]</td><td>Coding</td><td>H1</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245127 BBa_K245127]</td><td>Coding</td><td>GCN</td><td>99</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245128 BBa_K245128]</td><td>Coding</td><td>p53</td><td>90</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245129 BBa_K245129]</td><td>Coding</td><td>CutA1</td><td>333</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245130 BBa_K245130]</td><td>Coding</td><td>T1W_AEA</td><td>186</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245131 BBa_K245131]</td><td>Coding</td><td>ELST</td><td>150</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245132 BBa_K245132]</td><td>Coding</td><td>APH1</td><td>90</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245133 BBa_K245133]</td><td>Coding</td><td>APH</td><td>135</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245134 BBa_K245134]</td><td>Coding</td><td>BCR</td><td>108</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245135 BBa_K245135]</td><td>Coding</td><td>APHE</td><td>135</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245139 BBa_K245139]</td><td>Coding</td><td>RADA</td><td>66</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245140 BBa_K245140]</td><td>Coding</td><td>mNGF</td><td>669</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245141 BBa_K245141]</td><td>Coding</td><td>foldon</td><td>81</td></tr>
 +
<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245200 BBa_K245200]</td><td>Composite</td><td>P1-P4-H1</td><td>309</td></tr>
 +
</table>
 +
 +
}}

Revision as of 16:55, 21 October 2009


Designed BioBricks

We have designed four NEW functionalised linker-extension standard vectors. All together, 106 parts were generated all cloned into our NEW functionalised linker-extension standard vectors and forty-one were single coding, thirteen intermediates and forty-eight composite parts.

Plasmid backbones

Four NEW linker-extension standard vectors are constructed.


NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245005 BBa_K245005]Plasmid_BackboneBB-NIC-II-HisN2261
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245006 BBa_K245006]Plasmid_BackboneBB-NIC-II-NC2240
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]Plasmid_BackboneBB-NIC-II-HisC2167
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]Plasmid_BackboneBB-NIC-III-HisN2261


The coding sequences

The forty-one BioBrick coding sequences are prepared and are listed as (i) coiled-coil-forming segments, (ii) oligimerisation domains, (iii) functional domains and others.

Coiled-coil segments


NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245038 BBa_K245038]CodingNZ - cc homodimer87
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245039 BBa_K245039]CodingCZ90
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245118 BBa_K245118]CodingP199
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245119 BBa_K245119]CodingP299
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245120 BBa_K245120]CodingP399
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245121 BBa_K245121]CodingP499
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245122 BBa_K245122]CodingP599
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245123 BBa_K245123]CodingP699
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245124 BBa_K245124]CodingP799
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245125 BBa_K245125]CodingP899
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245126 BBa_K245126]CodingH199
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245127 BBa_K245127]CodingGCN99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245132 BBa_K245132]CodingAPH190
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245133 BBa_K245133]CodingAPH135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245134 BBa_K245134]CodingBCR108



Oligomerization domains

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245026 BBa_K245026]CodingTA63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245027 BBa_K245027]CodingTB63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245028 BBa_K245028]CodingTC63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245029 BBa_K245029]CodingT1W_DDD189
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245030 BBa_K245030]CodingT1W_KKK189
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245031 BBa_K245031]CodingT1W_EEE186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245042 BBa_K245042]CodingVASP135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245116 BBa_K245116]CodingGyrB657
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245117 BBa_K245117]CodingGyrB-SG663
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245128 BBa_K245128]Codingp5390
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245129 BBa_K245129]CodingCutA1333
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245130 BBa_K245130]CodingT1W_AEA186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245141 BBa_K245141]Codingfoldon81



Functional domains

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]CodingcYFP252
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]CodingnYFP462
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245024 BBa_K245024]CodingZ136
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245025 BBa_K245025]CodingAUP36
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245046 BBa_K245046]CodingccdB306
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245113 BBa_K245113]CodingKSI-DP378
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114]CodingLL37111
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]CodingKSI-DP-P2/33405
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245140 BBa_K245140]CodingmNGF669



Others

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245131 BBa_K245131]CodingELST150
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245139 BBa_K245139]CodingRADA66



The intermediate sequences

Thirteen BioBricks with intermediate parts are constructed.

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245048 BBa_K245048]IntermediateH1-P2204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245049 BBa_K245049]IntermediateKSI-DP-P1489
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245052 BBa_K245052]IntermediateGCN_P2204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245053 BBa_K245053]IntermediateP4-H1204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245054 BBa_K245054]IntermediateP2-H1204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245055 BBa_K245055]IntermediateP6-H1204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245056 BBa_K245056]IntermediateP3-P5204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245057 BBa_K245057]IntermediateP4-P2204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245059 BBa_K245059]IntermediateLL37-ELST267
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245060 BBa_K245060]IntermediateELST-ELST-ELST462
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245061 BBa_K245061]IntermediateELST-ELST306
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245096 BBa_K245096]IntermediateBCR-P2213
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245097 BBa_K245097]IntermediateAPH-P2240



Composite parts

The forty-eight BioBricks as composites are prepared, which are used in (i) polypeptide manufacturing, (ii) polypeptide membranes, (iii) coiled-coil polyhedra, (iv) regulated assembly and (v) functional biopolymers.



Polypeptide manufacturing




Polypeptide membrane

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]CodingKSI-DP-P2/33405
NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245091 BBa_K245091]Compositep53-APH1186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245092 BBa_K245092]Compositep53-APH231
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245093 BBa_K245093]Compositep53-BCR204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245099 BBa_K245099]CompositeAPH-p53231
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245100 BBa_K245100]CompositeBCR-p53204



Coiled-coil polyhedra

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245066 BBa_K245066]CompositeP1-GCN-P2309



Regulated assembly

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245098 BBa_K245098]CompositeGyrB-foldon744
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245050 BBa_K245050]CompositeGyrB-CutA1996
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245051 BBa_K245051]CompositeGyrB-SG-SG-CutA11002



Functional biopolymers

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245102 BBa_K245102]CompositeLL37-ELST-GCN-ELST-P2633
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245105 BBa_K245105]CompositeELST-ELST-ELST-mNGF1137



Slovenia 2009 iGEM Team Parts Sandbox



NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245005 BBa_K245005]Plasmid_BackboneBB-NIC-II-HisN2261
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245006 BBa_K245006]Plasmid_BackboneBB-NIC-II-NC2240
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]Plasmid_BackboneBB-NIC-II-HisC2167
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]Plasmid_BackboneBB-NIC-III-HisN2261
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245009 BBa_K245009]DNARFC 37 Scar Sequence6
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]CodingcYFP252
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]CodingnYFP462
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245024 BBa_K245024]CodingZ136
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245025 BBa_K245025]CodingAUP36
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245026 BBa_K245026]CodingTA63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245027 BBa_K245027]CodingTB63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245028 BBa_K245028]CodingTC63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245029 BBa_K245029]CodingT1W_DDD189
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245030 BBa_K245030]CodingT1W_KKK189
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245031 BBa_K245031]CodingT1W_EEE186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245038 BBa_K245038]CodingNZ - cc homodimer87
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245039 BBa_K245039]CodingCZ90
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245042 BBa_K245042]CodingVASP135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245046 BBa_K245046]CodingccdB306
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245048 BBa_K245048]IntermediateH1-P2204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245049 BBa_K245049]IntermediateKSI-DP-P1489
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245050 BBa_K245050]CompositeGyrB-CutA1996
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245051 BBa_K245051]CompositeGyrB-SG-SG-CutA11002
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245052 BBa_K245052]IntermediateGCN_P2204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245053 BBa_K245053]IntermediateP4-H1204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245054 BBa_K245054]IntermediateP2-H1204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245055 BBa_K245055]IntermediateP6-H1204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245056 BBa_K245056]IntermediateP3-P5204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245057 BBa_K245057]IntermediateP4-P2204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245058 BBa_K245058]CompositeKSI-DP-P6489
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245059 BBa_K245059]IntermediateLL37-ELST267
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245060 BBa_K245060]IntermediateELST-ELST-ELST462
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245061 BBa_K245061]IntermediateELST-ELST306
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245062 BBa_K245062]CompositeP1-H1-P2309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245063 BBa_K245063]CompositeKSI-DP-P1-H1-P2699
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245064 BBa_K245064]CompositeP1-BCR-P2318
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245065 BBa_K245065]CompositeP1-APH-P2345
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245066 BBa_K245066]CompositeP1-GCN-P2309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245067 BBa_K245067]CompositeP3-P4-H1309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245068 BBa_K245068]CompositeP1-P3-P5309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245069 BBa_K245069]CompositeKSI-DP-P1-P3-P5699
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245070 BBa_K245070]CompositeP6-P4-P2309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245071 BBa_K245071]CompositeKSI-DP-P6-P4-P2699
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245072 BBa_K245072]CompositeKSI-DP-LL37-ELST657
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245073 BBa_K245073]CompositeKSI-DP-LL37-ELST-ELST-ELST-ELST1125
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245074 BBa_K245074]CompositeKSI-DP-LL-37-ELST-GCN-ELST918
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245075 BBa_K245075]CompositeKSI-DP-EEE576
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245076 BBa_K245076]CompositeKSI_DP-LL37-ELST-P2-ELST918
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245077 BBa_K245077]CompositeKSI-DP-LL37-ELST-P4-ELST918
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245078 BBa_K245078]CompositeLL37-ELST-GCN-ELST528
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245079 BBa_K245079]CompositeGCN-ELST255
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245080 BBa_K245080]DNAscar Xba/Spe NgoMIV12
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245081 BBa_K245081]CompositeP2-ELST255
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245082 BBa_K245082]CompositeP4-ELST255
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245083 BBa_K245083]CompositeTA-P1168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245084 BBa_K245084]CompositeTB-P3168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245085 BBa_K245085]CompositeTC-P5168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245086 BBa_K245086]CompositeP2-TA168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245087 BBa_K245087]CompositeP4-TB168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245088 BBa_K245088]CompositeTC-P5168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245089 BBa_K245089]Compositefoldon-APH222
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245090 BBa_K245090]Compositefoldon-BCR195
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245091 BBa_K245091]Compositep53-APH1186
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245093 BBa_K245093]Compositep53-BCR204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245094 BBa_K245094]Compositefoldon-p53177
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245095 BBa_K245095]CompositeGyrB-p53753
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245096 BBa_K245096]IntermediateBCR-P2213
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245097 BBa_K245097]IntermediateAPH-P2240
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245100 BBa_K245100]CompositeBCR-p53204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245101 BBa_K245101]CompositeLL37-ELST-GCN-ELST-P1633
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245105 BBa_K245105]CompositeELST-ELST-ELST-mNGF1137
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245108 BBa_K245108]CompositeKSI-DP-P2/33-LL37-ELST-GCN-ELST945
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245113 BBa_K245113]CodingKSI-DP378
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114]CodingLL37111
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245116 BBa_K245116]CodingGyrB657
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245125 BBa_K245125]CodingP899
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245127 BBa_K245127]CodingGCN99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245128 BBa_K245128]Codingp5390
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245129 BBa_K245129]CodingCutA1333
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245131 BBa_K245131]CodingELST150
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245134 BBa_K245134]CodingBCR108
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245135 BBa_K245135]CodingAPHE135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245139 BBa_K245139]CodingRADA66
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245140 BBa_K245140]CodingmNGF669
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245141 BBa_K245141]Codingfoldon81
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245200 BBa_K245200]CompositeP1-P4-H1309



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