From 2009.igem.org

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Revision as of 18:14, 21 October 2009

Designed BioBricks

We have designed four NEW functionalised linker-extension standard vectors. All together, 106 parts were generated all cloned into our NEW functionalised linker-extension standard vectors and forty-one were single coding, thirteen intermediates and forty-eight composite parts.

Plasmid backbones

Four NEW linker-extension standard vectors are constructed.

Name	Type	Description	Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245005 BBa_K245005]	Plasmid_Backbone	BB-NIC-II-HisN	2261
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245006 BBa_K245006]	Plasmid_Backbone	BB-NIC-II-NC	2240
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]	Plasmid_Backbone	BB-NIC-II-HisC	2167
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]	Plasmid_Backbone	BB-NIC-III-HisN	2261

The coding sequences

The forty-one BioBrick coding sequences are prepared and are listed as (i) coiled-coil-forming segments, (ii) oligimerisation domains, (iii) functional domains and others.

Coiled-coil segments

Name	Type	Description	Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245038 BBa_K245038]	Coding	NZ - cc homodimer	87
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245039 BBa_K245039]	Coding	CZ	90
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245118 BBa_K245118]	Coding	P1	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245119 BBa_K245119]	Coding	P2	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245120 BBa_K245120]	Coding	P3	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245121 BBa_K245121]	Coding	P4	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245122 BBa_K245122]	Coding	P5	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245123 BBa_K245123]	Coding	P6	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245124 BBa_K245124]	Coding	P7	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245125 BBa_K245125]	Coding	P8	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245126 BBa_K245126]	Coding	H1	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245127 BBa_K245127]	Coding	GCN	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245132 BBa_K245132]	Coding	APH1	90
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245133 BBa_K245133]	Coding	APH	135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245134 BBa_K245134]	Coding	BCR	108

Oligomerization domains

Name	Type	Description	Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245026 BBa_K245026]	Coding	TA	63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245027 BBa_K245027]	Coding	TB	63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245028 BBa_K245028]	Coding	TC	63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245029 BBa_K245029]	Coding	T1W_DDD	189
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245030 BBa_K245030]	Coding	T1W_KKK	189
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245031 BBa_K245031]	Coding	T1W_EEE	186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245042 BBa_K245042]	Coding	VASP	135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245116 BBa_K245116]	Coding	GyrB	657
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245117 BBa_K245117]	Coding	GyrB-SG	663
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245128 BBa_K245128]	Coding	p53	90
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245129 BBa_K245129]	Coding	CutA1	333
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245130 BBa_K245130]	Coding	T1W_AEA	186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245141 BBa_K245141]	Coding	foldon	81

Functional domains

Name	Type	Description	Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]	Coding	cYFP	252
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]	Coding	nYFP	462
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245024 BBa_K245024]	Coding	Z1	36
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245025 BBa_K245025]	Coding	AUP	36
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245046 BBa_K245046]	Coding	ccdB	306
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245113 BBa_K245113]	Coding	KSI-DP	378
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114]	Coding	LL37	111
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]	Coding	KSI-DP-P2/33	405
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245140 BBa_K245140]	Coding	mNGF	669

Others

Name	Type	Description	Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245131 BBa_K245131]	Coding	ELST	150
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245139 BBa_K245139]	Coding	RADA	66

The intermediate sequences

Thirteen BioBricks with intermediate parts are constructed.

Name	Type	Description	Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245048 BBa_K245048]	Intermediate	H1-P2	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245049 BBa_K245049]	Intermediate	KSI-DP-P1	489
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245052 BBa_K245052]	Intermediate	GCN_P2	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245053 BBa_K245053]	Intermediate	P4-H1	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245054 BBa_K245054]	Intermediate	P2-H1	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245055 BBa_K245055]	Intermediate	P6-H1	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245056 BBa_K245056]	Intermediate	P3-P5	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245057 BBa_K245057]	Intermediate	P4-P2	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245059 BBa_K245059]	Intermediate	LL37-ELST	267
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245060 BBa_K245060]	Intermediate	ELST-ELST-ELST	462
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245061 BBa_K245061]	Intermediate	ELST-ELST	306
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245096 BBa_K245096]	Intermediate	BCR-P2	213
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245097 BBa_K245097]	Intermediate	APH-P2	240

Composite parts

The forty-eight BioBricks as composites are prepared, which are used in (i) polypeptide manufacturing, (ii) polypeptide membranes, (iii) coiled-coil polyhedra, (iv) regulated assembly and (v) functional biopolymers.

Polypeptide manufacturing

Name	Type	Description	Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]	Coding	KSI-DP-P2/33	405

Polypeptide membrane

Name	Type	Description	Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245091 BBa_K245091]	Composite	p53-APH1	186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245092 BBa_K245092]	Composite	p53-APH	231
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245093 BBa_K245093]	Composite	p53-BCR	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245099 BBa_K245099]	Composite	APH-p53	231
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245100 BBa_K245100]	Composite	BCR-p53	204

Coiled-coil polyhedra

Name	Type	Description	Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245066 BBa_K245066]	Composite	P1-GCN-P2	309

Regulated assembly

Name	Type	Description	Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245098 BBa_K245098]	Composite	GyrB-foldon	744
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245050 BBa_K245050]	Composite	GyrB-CutA1	996
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245051 BBa_K245051]	Composite	GyrB-SG-SG-CutA1	1002

Functional biopolymers

Name	Type	Description	Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245102 BBa_K245102]	Composite	LL37-ELST-GCN-ELST-P2	633
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245105 BBa_K245105]	Composite	ELST-ELST-ELST-mNGF	1137

Slovenia 2009 iGEM Team Parts Sandbox

Name	Type	Description	Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245005 BBa_K245005]	Plasmid_Backbone	BB-NIC-II-HisN	2261
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245006 BBa_K245006]	Plasmid_Backbone	BB-NIC-II-NC	2240
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]	Plasmid_Backbone	BB-NIC-II-HisC	2167
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]	Plasmid_Backbone	BB-NIC-III-HisN	2261
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245009 BBa_K245009]	DNA	RFC 37 Scar Sequence	6
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]	Coding	cYFP	252
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]	Coding	nYFP	462
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245024 BBa_K245024]	Coding	Z1	36
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245025 BBa_K245025]	Coding	AUP	36
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245026 BBa_K245026]	Coding	TA	63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245027 BBa_K245027]	Coding	TB	63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245028 BBa_K245028]	Coding	TC	63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245029 BBa_K245029]	Coding	T1W_DDD	189
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245030 BBa_K245030]	Coding	T1W_KKK	189
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245031 BBa_K245031]	Coding	T1W_EEE	186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245038 BBa_K245038]	Coding	NZ - cc homodimer	87
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245039 BBa_K245039]	Coding	CZ	90
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245042 BBa_K245042]	Coding	VASP	135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245046 BBa_K245046]	Coding	ccdB	306
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245048 BBa_K245048]	Intermediate	H1-P2	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245049 BBa_K245049]	Intermediate	KSI-DP-P1	489
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245050 BBa_K245050]	Composite	GyrB-CutA1	996
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245051 BBa_K245051]	Composite	GyrB-SG-SG-CutA1	1002
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245052 BBa_K245052]	Intermediate	GCN_P2	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245053 BBa_K245053]	Intermediate	P4-H1	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245054 BBa_K245054]	Intermediate	P2-H1	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245055 BBa_K245055]	Intermediate	P6-H1	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245056 BBa_K245056]	Intermediate	P3-P5	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245057 BBa_K245057]	Intermediate	P4-P2	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245058 BBa_K245058]	Composite	KSI-DP-P6	489
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245059 BBa_K245059]	Intermediate	LL37-ELST	267
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245060 BBa_K245060]	Intermediate	ELST-ELST-ELST	462
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245061 BBa_K245061]	Intermediate	ELST-ELST	306
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245062 BBa_K245062]	Composite	P1-H1-P2	309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245063 BBa_K245063]	Composite	KSI-DP-P1-H1-P2	699
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245064 BBa_K245064]	Composite	P1-BCR-P2	318
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245065 BBa_K245065]	Composite	P1-APH-P2	345
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245066 BBa_K245066]	Composite	P1-GCN-P2	309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245067 BBa_K245067]	Composite	P3-P4-H1	309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245068 BBa_K245068]	Composite	P1-P3-P5	309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245069 BBa_K245069]	Composite	KSI-DP-P1-P3-P5	699
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245070 BBa_K245070]	Composite	P6-P4-P2	309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245071 BBa_K245071]	Composite	KSI-DP-P6-P4-P2	699
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245072 BBa_K245072]	Composite	KSI-DP-LL37-ELST	657
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245073 BBa_K245073]	Composite	KSI-DP-LL37-ELST-ELST-ELST-ELST	1125
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245074 BBa_K245074]	Composite	KSI-DP-LL-37-ELST-GCN-ELST	918
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245075 BBa_K245075]	Composite	KSI-DP-EEE	576
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245076 BBa_K245076]	Composite	KSI_DP-LL37-ELST-P2-ELST	918
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245077 BBa_K245077]	Composite	KSI-DP-LL37-ELST-P4-ELST	918
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245078 BBa_K245078]	Composite	LL37-ELST-GCN-ELST	528
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245079 BBa_K245079]	Composite	GCN-ELST	255
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245080 BBa_K245080]	DNA	scar Xba/Spe NgoMIV	12
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245081 BBa_K245081]	Composite	P2-ELST	255
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245082 BBa_K245082]	Composite	P4-ELST	255
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245083 BBa_K245083]	Composite	TA-P1	168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245084 BBa_K245084]	Composite	TB-P3	168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245085 BBa_K245085]	Composite	TC-P5	168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245086 BBa_K245086]	Composite	P2-TA	168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245087 BBa_K245087]	Composite	P4-TB	168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245088 BBa_K245088]	Composite	TC-P5	168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245089 BBa_K245089]	Composite	foldon-APH	222
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245090 BBa_K245090]	Composite	foldon-BCR	195
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245091 BBa_K245091]	Composite	p53-APH1	186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245092 BBa_K245092]	Composite	p53-APH	231
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245093 BBa_K245093]	Composite	p53-BCR	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245094 BBa_K245094]	Composite	foldon-p53	177
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245095 BBa_K245095]	Composite	GyrB-p53	753
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245096 BBa_K245096]	Intermediate	BCR-P2	213
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245097 BBa_K245097]	Intermediate	APH-P2	240
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245098 BBa_K245098]	Composite	GyrB-foldon	744
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245099 BBa_K245099]	Composite	APH-p53	231
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245100 BBa_K245100]	Composite	BCR-p53	204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245101 BBa_K245101]	Composite	LL37-ELST-GCN-ELST-P1	633
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245102 BBa_K245102]	Composite	LL37-ELST-GCN-ELST-P2	633
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245105 BBa_K245105]	Composite	ELST-ELST-ELST-mNGF	1137
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245106 BBa_K245106]	Composite	RADA-mNGF	741
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245107 BBa_K245107]	Composite	LL37-ELST-GCN-ELST-mNGF	1203
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245108 BBa_K245108]	Composite	KSI-DP-P2/33-LL37-ELST-GCN-ELST	945
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245111 BBa_K245111]	Composite	P2-P6-H1	309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245112 BBa_K245112]	Composite	P5-P2-H1	309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245113 BBa_K245113]	Coding	KSI-DP	378
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114]	Coding	LL37	111
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]	Coding	KSI-DP-P2/33	405
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245116 BBa_K245116]	Coding	GyrB	657
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245117 BBa_K245117]	Coding	GyrB-SG	663
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245118 BBa_K245118]	Coding	P1	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245119 BBa_K245119]	Coding	P2	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245120 BBa_K245120]	Coding	P3	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245121 BBa_K245121]	Coding	P4	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245122 BBa_K245122]	Coding	P5	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245123 BBa_K245123]	Coding	P6	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245124 BBa_K245124]	Coding	P7	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245125 BBa_K245125]	Coding	P8	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245126 BBa_K245126]	Coding	H1	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245127 BBa_K245127]	Coding	GCN	99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245128 BBa_K245128]	Coding	p53	90
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245129 BBa_K245129]	Coding	CutA1	333
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245130 BBa_K245130]	Coding	T1W_AEA	186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245131 BBa_K245131]	Coding	ELST	150
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245132 BBa_K245132]	Coding	APH1	90
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245133 BBa_K245133]	Coding	APH	135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245134 BBa_K245134]	Coding	BCR	108
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245135 BBa_K245135]	Coding	APHE	135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245139 BBa_K245139]	Coding	RADA	66
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245140 BBa_K245140]	Coding	mNGF	669
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245141 BBa_K245141]	Coding	foldon	81
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245200 BBa_K245200]	Composite	P1-P4-H1	309

@@ Line 167: / Line 167: @@
 <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]</td><td>Plasmid_Backbone</td><td>BB-NIC-II-HisC</td><td>2167</td></tr>
 <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]</td><td>Plasmid_Backbone</td><td>BB-NIC-III-HisN</td><td>2261</td></tr>
-<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245009 BBa_K245009]</td><td>DNA</td><td>RFC 37 Scar Sequence</td><td>6</td></tr>
+<tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245009 BBa_K245009]</td><td>DNA</td><td><nowiki>RFC</nowiki> 37 Scar Sequence</td><td>6</td></tr>
 <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]</td><td>Coding</td><td>cYFP</td><td>252</td></tr>
 <tr><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]</td><td>Coding</td><td>nYFP</td><td>462 </td></tr>

Deposited BioBricks.html