Team:UNICAMP-Brazil/Notebooks/October 19

From 2009.igem.org

(Difference between revisions)
(→YeastGuard)
Line 3: Line 3:
__NOTOC__  
__NOTOC__  
 +
 +
==''' ColiGuard '''==
 +
 +
==== PY Promoter - Conjugation test ====
 +
 +
''Fabi and Léo''

Revision as of 20:35, 21 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

ColiGuard

PY Promoter - Conjugation test

Fabi and Léo


YeastGuard

New strategy: pGEM

  • We sent the pDLD+lysozyme device to iGEM today. =)

Yeast experiments

  • There are plenty of yeast colonies in the pDLD+Lys and Adh1+Lys plates. =)

  • We did PCR with 30 yeast colonies transformed with Adh1+Lysozyme and pDLD+Lysozyme. Unfortunately none of the colonies had the correct vector =/

Adh1+lis2.jpg
PDLD+lis.jpg

  • We have no time to repeat the transformations =( Our hope is that there has been a problem to the PCR reaction and that the parts are correctly connected to the YEP vector. So we decided to go on with the transformed yeasts we have.

  • At night we find plenty yeast colonies in the pDLD+YFP and Adh1+YFP plates.

  • Considering our lack of time we decided to collect a pool of yeast colonies for each construction and inoculate in liquid media. This strategy was adopted for both constructions (pDLD+YFP and Adh1+YFP). These pre inocula will be used for induction tests of Lysozyme tomorrow.


Raíssa and Taís




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_19"