"
Team:Newcastle/Promoter Library
From 2009.igem.org
(Difference between revisions)
(→Design) |
(→Design) |
||
| Line 12: | Line 12: | ||
==Design== | ==Design== | ||
| + | |||
| + | Template used to create the promoter sequences: | ||
| + | '''Prefix...UpSpacer...-35...PromSpacer...-10...DownSpacer..Suffix''' | ||
[[Image:Team_Newcastle_iGEM_Promoter_Library1.png|500px]] | [[Image:Team_Newcastle_iGEM_Promoter_Library1.png|500px]] | ||
| Line 17: | Line 20: | ||
[[Image:Team_Newcastle_iGEM_Promoter_Library2.png|500px]] | [[Image:Team_Newcastle_iGEM_Promoter_Library2.png|500px]] | ||
| - | + | ||
| - | + | ||
* '''BBPrefix''' - StandardBioBrickPrefix EcoR1 and Xba1 - gaattcgcggccgcttctagag | * '''BBPrefix''' - StandardBioBrickPrefix EcoR1 and Xba1 - gaattcgcggccgcttctagag | ||
* '''UpSpacer''' - attta - consensus sigA 5 bases 5' | * '''UpSpacer''' - attta - consensus sigA 5 bases 5' | ||
Revision as of 20:55, 21 October 2009
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Promoter Library
Introduction
As a part of our project we wish to create a library of SigmaA promoters and characterise them based on their stregnths.
Novelty in this sub-project
A library of promoters with different strengths would be very useful for engineering biological devices. Promoters' strength can be compared with their PoPS values. However measuring the PoPS is not a trivial process. We can instead meausure their strengths relatively.
Design
Template used to create the promoter sequences: Prefix...UpSpacer...-35...PromSpacer...-10...DownSpacer..Suffix
- BBPrefix - StandardBioBrickPrefix EcoR1 and Xba1 - gaattcgcggccgcttctagag
- UpSpacer - attta - consensus sigA 5 bases 5'
- -35 - TTGACA
- PromSpacer - length 17 - consensus ttttatttaaattatga
- -10 - TATAAT
- DownSpacer (from ackA)- ggaaaag
- BBSuffix - Standard BioBrick suffix Spe1 and Pst1 - tactagtagcggccgctgcag
Variances
- BBPrefix...UpSpacer...N-35... PromSpacer...N-10...DownSpacer..BBSuffix
- BBPrefix...UpSpacerNN...-35...NNPromSpacerNN...-10...NNDownSpacer..BBSuffix
- BBPrefix...UpSpacer...-35... 18N’s...-10...DownSpacer..BBSuffix
Variance 1
Variance 2
Variance 3
Lab Work Strategies
Other Presentations and Diagrams
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
