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Team:UNICAMP-Brazil/Notebooks/October 20
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====Yeast experiments: Lysozyme==== | ====Yeast experiments: Lysozyme==== | ||
| - | *<p style=”text-align:justify;”>Today we began | + | *<p style=”text-align:justify;”>Today we began the tests with the yeasts transformed with the following constructions: YEP+pDLD-Lysozyme and YEP+Adh1-Lysozyme.</p> |
*<p style=”text-align:justify;”>We inoculated the yeast pre inocula in 3 types of media: YNB Glucose Ura-, YNB Ethanol Ura- and YNB Glucose Ura+. The inocula grew for 4 hours in 30ºC and 150rpm before the induction with lactic acid. Four concentrations of lactic acid were used in the induction.</p> | *<p style=”text-align:justify;”>We inoculated the yeast pre inocula in 3 types of media: YNB Glucose Ura-, YNB Ethanol Ura- and YNB Glucose Ura+. The inocula grew for 4 hours in 30ºC and 150rpm before the induction with lactic acid. Four concentrations of lactic acid were used in the induction.</p> | ||
Revision as of 22:07, 21 October 2009
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